Fatty Acid Distribution in Bacterial Phospholipids. The Specificity of the Cyclopropane Synthetase Reaction

John G. Hildebrand, John H. Law

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The distribution of fatty acids in purified phospholipids isolated from several different bacteria has been examined by use of the specific phospholipase A of snake venom. In general the distribution is in accord with that found elsewhere in nature; the unsaturated acids are found in the β position and the saturated acids in the γ position. The distribution of cyclopropane fatty acids follows closely that of the unsaturated fatty acids. A notable exception was encountered with the phosphatidylethanolamine of Clostridium butyricum, in which unsaturated and cyclopropane fatty acids were found in more abundance in the γ position. The specificity of the C. butyricum cyclopropane synthetase reaction has been examined by employing as substrate a phosphatidylethanolamine with a known distribution of unsaturated fatty acids and analyzing the distribution of cyclopropane fatty acids in the phospholipid produced by the enzyme reaction. These experiments indicate that the enzyme has a definite, but not absolute, specificity for an unsaturated fatty acid in the γ position.

Original languageEnglish (US)
Pages (from-to)1304-1308
Number of pages5
JournalBiochemistry
Volume3
Issue number9
DOIs
StatePublished - Sep 1 1964
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Fatty Acid Distribution in Bacterial Phospholipids. The Specificity of the Cyclopropane Synthetase Reaction'. Together they form a unique fingerprint.

  • Cite this