Fatty acid distribution in bacterial phospholipids. The specificity of the cyclopropane synthetase reaction

John G. Hildebrand, John H. Law

Research output: Research - peer-reviewArticle

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Abstract

The distribution of fatty acids in purified phospholipids isolated from several different bacteria has been examined by use of the specific phospholipase A of snake venom. In general the distribution is in accord with that found elsewhere in nature; the unsaturated acids are found in the β position and the saturated acids in the γ position. The distribution of cyclopropane fatty acids follows closely that of the unsaturated fatty acids. A notable exception was encountered with the phosphatidylethanolamine of Clostridium butyricum, in which unsaturated and cyclopropane fatty acids were found in more abundance in the γ position. The specificity of the C. butyricum cyclopropane synthetase reaction has been examined by employing as substrate a phosphatidylethanolamine with a known distribution of unsaturated fatty acids and analyzing the distribution of cyclopropane fatty acids in the phospholipid produced by the enzyme reaction. These experiments indicate that the enzyme has a definite, but not absolute, specificity for an unsaturated fatty acid in the γ position.

LanguageEnglish (US)
Pages1304-1308
Number of pages5
JournalBiochemistry
Volume3
Issue number9
StatePublished - 1964
Externally publishedYes

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Unsaturated Fatty Acids
Phospholipids
Fatty Acids
cyclopropane synthetase
cyclopropane fatty acids
Clostridium butyricum
Acids
Enzymes
phosphatidylethanolamine
Snake Venoms
Phospholipases A
Bacteria
Clostridium
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fatty acid distribution in bacterial phospholipids. The specificity of the cyclopropane synthetase reaction. / Hildebrand, John G.; Law, John H.

In: Biochemistry, Vol. 3, No. 9, 1964, p. 1304-1308.

Research output: Research - peer-reviewArticle

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