Fecal NIRS: Detection of tick infestations in cattle and horses

Douglas R Tolleson, P. D. Teel, J. W. Stuth, O. F. Strey, T. H. Welsh, G. E. Carstens

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Anti-tick treatments are often applied concurrent to routine livestock management practices with little regard to actual infestation levels. Prescription treatments against ticks on grazing cattle would be facilitated by non-invasive detection methods. One such method is fecal near infrared spectroscopy (NIRS). Four studies utilizing cattle (Bos spp.) and one with horses (Equus caballus) fed varying diets and infested with either Amblyomma americanum, A. maculatum, A. cajennense or Dermacentor albipictus were conducted to determine the ability of fecal NIRS to identify samples from animals with (High stress) and without (Low stress) a tick burden. Discriminant analysis of each individual trial resulted in R2 > 0.80. Similar analyses utilizing all combinations of four studies, predicting group membership in the remaining study, yielded R2 > 0.80, but correct determinations for Low and High tick stress samples of only 53.4 and 60.1%, respectively. All five trials were combined and a random 10 or 25% of the samples were removed from the calibration. As in the previous calibrations, a high degree of discrimination was achieved (R2 > 0.89). The validation samples were correctly identified at 91.7% for Low stress and 96.3% for High stress, respectively. Difficulties in detecting differences in fecal samples due to confounding effects of trial were overcome by combining calibration sets. Overall, differences in fecal NIR spectra apparently due to tick stress were accurately detected across diet, host species, and tick species.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalVeterinary Parasitology
Volume144
Issue number1-2
DOIs
StatePublished - Mar 15 2007
Externally publishedYes

Fingerprint

Tick Infestations
tick infestations
Near-Infrared Spectroscopy
near-infrared spectroscopy
Ticks
Horses
ticks
horses
cattle
Calibration
calibration
sampling
Dermacentor albipictus
Amblyomma maculatum
Amblyomma cajennense
Dermacentor
Bos
Diet
Amblyomma americanum
Practice Management

Keywords

  • Amblyomma
  • Cattle
  • Discriminant analyses
  • Fecal NIRS
  • Horses
  • IPM
  • Tick stress
  • Validation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

Tolleson, D. R., Teel, P. D., Stuth, J. W., Strey, O. F., Welsh, T. H., & Carstens, G. E. (2007). Fecal NIRS: Detection of tick infestations in cattle and horses. Veterinary Parasitology, 144(1-2), 146-152. https://doi.org/10.1016/j.vetpar.2006.09.018

Fecal NIRS : Detection of tick infestations in cattle and horses. / Tolleson, Douglas R; Teel, P. D.; Stuth, J. W.; Strey, O. F.; Welsh, T. H.; Carstens, G. E.

In: Veterinary Parasitology, Vol. 144, No. 1-2, 15.03.2007, p. 146-152.

Research output: Contribution to journalArticle

Tolleson, DR, Teel, PD, Stuth, JW, Strey, OF, Welsh, TH & Carstens, GE 2007, 'Fecal NIRS: Detection of tick infestations in cattle and horses', Veterinary Parasitology, vol. 144, no. 1-2, pp. 146-152. https://doi.org/10.1016/j.vetpar.2006.09.018
Tolleson, Douglas R ; Teel, P. D. ; Stuth, J. W. ; Strey, O. F. ; Welsh, T. H. ; Carstens, G. E. / Fecal NIRS : Detection of tick infestations in cattle and horses. In: Veterinary Parasitology. 2007 ; Vol. 144, No. 1-2. pp. 146-152.
@article{0618a8af3f924f5fb8fe89c4210d6f98,
title = "Fecal NIRS: Detection of tick infestations in cattle and horses",
abstract = "Anti-tick treatments are often applied concurrent to routine livestock management practices with little regard to actual infestation levels. Prescription treatments against ticks on grazing cattle would be facilitated by non-invasive detection methods. One such method is fecal near infrared spectroscopy (NIRS). Four studies utilizing cattle (Bos spp.) and one with horses (Equus caballus) fed varying diets and infested with either Amblyomma americanum, A. maculatum, A. cajennense or Dermacentor albipictus were conducted to determine the ability of fecal NIRS to identify samples from animals with (High stress) and without (Low stress) a tick burden. Discriminant analysis of each individual trial resulted in R2 > 0.80. Similar analyses utilizing all combinations of four studies, predicting group membership in the remaining study, yielded R2 > 0.80, but correct determinations for Low and High tick stress samples of only 53.4 and 60.1{\%}, respectively. All five trials were combined and a random 10 or 25{\%} of the samples were removed from the calibration. As in the previous calibrations, a high degree of discrimination was achieved (R2 > 0.89). The validation samples were correctly identified at 91.7{\%} for Low stress and 96.3{\%} for High stress, respectively. Difficulties in detecting differences in fecal samples due to confounding effects of trial were overcome by combining calibration sets. Overall, differences in fecal NIR spectra apparently due to tick stress were accurately detected across diet, host species, and tick species.",
keywords = "Amblyomma, Cattle, Discriminant analyses, Fecal NIRS, Horses, IPM, Tick stress, Validation",
author = "Tolleson, {Douglas R} and Teel, {P. D.} and Stuth, {J. W.} and Strey, {O. F.} and Welsh, {T. H.} and Carstens, {G. E.}",
year = "2007",
month = "3",
day = "15",
doi = "10.1016/j.vetpar.2006.09.018",
language = "English (US)",
volume = "144",
pages = "146--152",
journal = "Veterinary Parasitology: Regional Studies and Reports",
issn = "0304-4017",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Fecal NIRS

T2 - Detection of tick infestations in cattle and horses

AU - Tolleson, Douglas R

AU - Teel, P. D.

AU - Stuth, J. W.

AU - Strey, O. F.

AU - Welsh, T. H.

AU - Carstens, G. E.

PY - 2007/3/15

Y1 - 2007/3/15

N2 - Anti-tick treatments are often applied concurrent to routine livestock management practices with little regard to actual infestation levels. Prescription treatments against ticks on grazing cattle would be facilitated by non-invasive detection methods. One such method is fecal near infrared spectroscopy (NIRS). Four studies utilizing cattle (Bos spp.) and one with horses (Equus caballus) fed varying diets and infested with either Amblyomma americanum, A. maculatum, A. cajennense or Dermacentor albipictus were conducted to determine the ability of fecal NIRS to identify samples from animals with (High stress) and without (Low stress) a tick burden. Discriminant analysis of each individual trial resulted in R2 > 0.80. Similar analyses utilizing all combinations of four studies, predicting group membership in the remaining study, yielded R2 > 0.80, but correct determinations for Low and High tick stress samples of only 53.4 and 60.1%, respectively. All five trials were combined and a random 10 or 25% of the samples were removed from the calibration. As in the previous calibrations, a high degree of discrimination was achieved (R2 > 0.89). The validation samples were correctly identified at 91.7% for Low stress and 96.3% for High stress, respectively. Difficulties in detecting differences in fecal samples due to confounding effects of trial were overcome by combining calibration sets. Overall, differences in fecal NIR spectra apparently due to tick stress were accurately detected across diet, host species, and tick species.

AB - Anti-tick treatments are often applied concurrent to routine livestock management practices with little regard to actual infestation levels. Prescription treatments against ticks on grazing cattle would be facilitated by non-invasive detection methods. One such method is fecal near infrared spectroscopy (NIRS). Four studies utilizing cattle (Bos spp.) and one with horses (Equus caballus) fed varying diets and infested with either Amblyomma americanum, A. maculatum, A. cajennense or Dermacentor albipictus were conducted to determine the ability of fecal NIRS to identify samples from animals with (High stress) and without (Low stress) a tick burden. Discriminant analysis of each individual trial resulted in R2 > 0.80. Similar analyses utilizing all combinations of four studies, predicting group membership in the remaining study, yielded R2 > 0.80, but correct determinations for Low and High tick stress samples of only 53.4 and 60.1%, respectively. All five trials were combined and a random 10 or 25% of the samples were removed from the calibration. As in the previous calibrations, a high degree of discrimination was achieved (R2 > 0.89). The validation samples were correctly identified at 91.7% for Low stress and 96.3% for High stress, respectively. Difficulties in detecting differences in fecal samples due to confounding effects of trial were overcome by combining calibration sets. Overall, differences in fecal NIR spectra apparently due to tick stress were accurately detected across diet, host species, and tick species.

KW - Amblyomma

KW - Cattle

KW - Discriminant analyses

KW - Fecal NIRS

KW - Horses

KW - IPM

KW - Tick stress

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=33846850624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846850624&partnerID=8YFLogxK

U2 - 10.1016/j.vetpar.2006.09.018

DO - 10.1016/j.vetpar.2006.09.018

M3 - Article

C2 - 17097809

AN - SCOPUS:33846850624

VL - 144

SP - 146

EP - 152

JO - Veterinary Parasitology: Regional Studies and Reports

JF - Veterinary Parasitology: Regional Studies and Reports

SN - 0304-4017

IS - 1-2

ER -