Fetal adrenal demedullation lowers circulating norepinephrine and attenuates growth restriction but not reduction of endocrine cell mass in an ovine model of intrauterine growth restriction

Melissa A. Davis, Antoni R. Macko, Leah V. Steyn, Miranda J. Anderson, Sean W Limesand

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14 Citations (Scopus)

Abstract

Placental insufficiency is associated with fetal hypoglycemia, hypoxemia, and elevated plasma norepinephrine (NE) that become increasingly pronounced throughout the third trimester and contribute to intrauterine growth restriction (IUGR). This study evaluated the effect of fetal adrenal demedullation (AD) on growth and pancreatic endocrine cell mass. Placental insufficiency-induced IUGR was created by exposing pregnant ewes to elevated ambient temperatures during mid-gestation. Treatment groups consisted of control and IUGR fetuses with either surgical sham or AD at 98 days gestational age (dGA; term = 147 dGA), a time-point that precedes IUGR. Samples were collected at 134 dGA. IUGR-sham fetuses were hypoxemic, hypoglycemic, and hypoinsulinemic, and values were similar in IUGR-AD fetuses. Plasma NE concentrations were ~5-fold greater in IUGR-sham compared to control-sham, control-AD, and IUGR-AD fetuses. IUGR-sham and IUGR-AD fetuses weighed less than controls. Compared to IUGR-sham fetuses, IUGR-AD fetuses weighed more and asymmetrical organ growth was absent. Pancreatic β-cell mass and α-cell mass were lower in both IUGR-sham and IUGR-AD fetuses compared to controls, however, pancreatic endocrine cell mass relative to fetal mass was lower in IUGR-AD fetuses. These findings indicate that NE, independently of hypoxemia, hypoglycemia and hypoinsulinemia, influence growth and asymmetry of growth but not pancreatic endocrine cell mass in IUGR fetuses.

Original languageEnglish (US)
Pages (from-to)500-516
Number of pages17
JournalNutrients
Volume7
Issue number1
DOIs
StatePublished - Jan 9 2015

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Endocrine Cells
norepinephrine
fetus
Sheep
Norepinephrine
sheep
Growth
Fetus
cells
hypoglycemia
hypoxia
Placental Insufficiency
gestational age
Hypoglycemia
ewes
ambient temperature
pregnancy

Keywords

  • Catecholamines
  • Fetal growth
  • Insulin
  • Pregnancy

ASJC Scopus subject areas

  • Food Science

Cite this

Fetal adrenal demedullation lowers circulating norepinephrine and attenuates growth restriction but not reduction of endocrine cell mass in an ovine model of intrauterine growth restriction. / Davis, Melissa A.; Macko, Antoni R.; Steyn, Leah V.; Anderson, Miranda J.; Limesand, Sean W.

In: Nutrients, Vol. 7, No. 1, 09.01.2015, p. 500-516.

Research output: Contribution to journalArticle

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