Fever-range hyperthermia dynamically regulates lymphocyte delivery to high endothelial venules

Sharon S. Evans, Wan Chao Wang, Mark D. Bain, Randy M Burd, Julie R. Ostberg, Elizabeth A. Repasky

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Fever is associated with increased survival during acute infection, although its mechanism of action is largely unknown. This study found evidence of an unexpectedly integrated mechanism by which fever-range temperatures stimulate lymphocyte homing to secondary lymphoid tissues by increasing L-selectin and α4β7 integrin-dependent adhesive interactions between circulating lymphocytes and specialized high endothelial venules (HEV). Exposure of splenic lymphocytes in vivo to fever-like whole-body hyperthermia (WBH; 39.8 ± 0.2°C for 6 hours) stimulated both L-selectin and α4β7 integrin-dependent adhesion of lymphocytes to HEV under shear conditions in lymph nodes and Peyer patches. The adhesiveness of HEV ligands for L-selectin and α4β7 integrin (ie, peripheral lymph node addressin and mucosal addressin cell adhesion molecule-1) also increased during WBH or febrile responses associated with lipopolysaccharide-induced or turpentine-induced inflammation. Similar increases in HEV adhesion occurred during hyperthermia treatment of lymph node and Peyer patch organ cultures in vitro, indicating that the local lymphoid tissue microenvironment is sufficient for the hyperthermia response. In contrast, WBH did not augment adhesion in squamous endothelium of nonlymphoid tissues. Analysis of homing of α4β7hi L-selectinlo murine TK1 cells and L-selectinhi α4β7 integrin-negative 300.19/L-selectin transfectant cells showed that fever-range temperatures caused a 3- to 4-fold increase in L-selectin and α4β7 integrin-dependent trafficking to secondary lymphoid tissues. Thus, enhanced lymphocyte delivery to HEV by febrile temperatures through bimodal regulation of lymphocyte and endothelial adhesion provides a novel mechanism to promote immune surveillance.

Original languageEnglish (US)
Pages (from-to)2727-2733
Number of pages7
JournalBlood
Volume97
Issue number9
DOIs
StatePublished - May 1 2001
Externally publishedYes

Fingerprint

Venules
Lymphocytes
L-Selectin
Integrins
Fever
Adhesion
Tissue
Lymphoid Tissue
Peyer's Patches
Turpentine
Temperature
Cell Adhesion Molecules
Lymph Nodes
Lipopolysaccharides
Adhesiveness
Adhesives
Organ Culture Techniques
Ligands
Endothelium
Inflammation

ASJC Scopus subject areas

  • Hematology

Cite this

Evans, S. S., Wang, W. C., Bain, M. D., Burd, R. M., Ostberg, J. R., & Repasky, E. A. (2001). Fever-range hyperthermia dynamically regulates lymphocyte delivery to high endothelial venules. Blood, 97(9), 2727-2733. https://doi.org/10.1182/blood.V97.9.2727

Fever-range hyperthermia dynamically regulates lymphocyte delivery to high endothelial venules. / Evans, Sharon S.; Wang, Wan Chao; Bain, Mark D.; Burd, Randy M; Ostberg, Julie R.; Repasky, Elizabeth A.

In: Blood, Vol. 97, No. 9, 01.05.2001, p. 2727-2733.

Research output: Contribution to journalArticle

Evans, SS, Wang, WC, Bain, MD, Burd, RM, Ostberg, JR & Repasky, EA 2001, 'Fever-range hyperthermia dynamically regulates lymphocyte delivery to high endothelial venules', Blood, vol. 97, no. 9, pp. 2727-2733. https://doi.org/10.1182/blood.V97.9.2727
Evans, Sharon S. ; Wang, Wan Chao ; Bain, Mark D. ; Burd, Randy M ; Ostberg, Julie R. ; Repasky, Elizabeth A. / Fever-range hyperthermia dynamically regulates lymphocyte delivery to high endothelial venules. In: Blood. 2001 ; Vol. 97, No. 9. pp. 2727-2733.
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