Fiber optic probe for polarized reflectance spectroscopy in vivo: Design and performance

Alexey Myakov, Linda Nieman, Lorenz Wicky, Urs Utzinger, Rebecca Richards-Kortum, Konstantin Sokolov

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

We present the design and construction of a fiber optic probe for elastic light scattering spectroscopy in vivo with polarized excitation and polarization sensitive detection. The performance of the fiber probe is evaluated using a suspension of polystyrene spheres placed atop a diffusely scattering substrate, and it demonstrates that the size-dependent characteristics of the scatterers can be extracted in the presence of a highly diffusely scattering background using a linear combination of forward and backward Mie scattering components of the scatterers. Subsequently, Mie theory calculations are performed over a broad range of diagnostically relevant parameters of nuclei-mean diameter, size distribution, and relative refractive index-to understand how the polarized reflectance measurements with the fiber probe can be used to extract morphological information about epithelial tissue. Finally, the feasibility of in vivo measurements with the fiber optic based polarization sensitive light scattering spectroscopy is demonstrated.

Original languageEnglish (US)
Pages (from-to)388-397
Number of pages10
JournalJournal of biomedical optics
Volume7
Issue number3
DOIs
StatePublished - Jul 1 2002
Externally publishedYes

Keywords

  • Cancer diagnosis
  • Elastic light scattering
  • Polarized light
  • Reflectance spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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