Fibronectin stimulates TRPV1 translocation in primary sensory neurons

Nathaniel A. Jeske, Amol M. Patwardhan, Michael A. Henry, Stephen B. Milam

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Extracellular matrix (ECM) molecules are highly variable in their composition and receptor recognition. Their ubiquitous expression profile has been linked to roles in cell growth, differentiation, and survival. Recent work has identified certain ECM molecules that serve as dynamic signal modulators, versus the more-recognized role of chronic modulation of signal transduction. In this study, we investigated the role that fibronectin (FN) plays in the dynamic modulation of transient receptor potential family V type 1 receptor (TRPV1) translocation to the plasma membrane in trigeminal ganglia (TG) sensory neurons. Confocal immunofluorescence analyses identify co-expression of the TRPV1 receptor with integrin subunits that bind FN. TG neurons cultured upon or treated with FN experienced a leftward shift in the EC50 of capsaicin-stimulated neuropeptide release. This FN-induced increase in TRPV1 sensitivity to activation is coupled by an increase in plasma membrane expression of TRPV1, as well as an increase in tyrosine phosphorylation of TRPV1 in TG neurons. Furthermore, TG neurons cultured on FN demonstrated an increase in capsaicin-mediated Ca2+ accumulation relative to neurons cultured on poly-d-lysine. Data presented from these studies indicate that FN stimulates tyrosine-phosphorylation-dependent translocation of the TRPV1 receptor to the plasma membrane, identifying FN as a critical component of the ECM capable of sensory neuron sensitization.

Original languageEnglish (US)
Pages (from-to)591-600
Number of pages10
JournalJournal of neurochemistry
Volume108
Issue number3
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

Keywords

  • Fibronectin
  • Integrin
  • Pain
  • Src
  • Transient receptor potential family V type 1 receptor

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Fibronectin stimulates TRPV1 translocation in primary sensory neurons'. Together they form a unique fingerprint.

  • Cite this