Field performance of a genetically engineered strain of pink bollworm

Gregory S. Simmons, Andrew R. McKemey, Neil I. Morrison, Sinead O'Connell, Bruce E Tabashnik, John Claus, Guoliang Fu, Guolei Tang, Mickey Sledge, Adam S. Walker, Caroline E. Phillips, Ernie D. Miller, Robert I. Rose, Robert T. Staten, Christl A. Donnelly, Luke Alphey

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Pest insects harm crops, livestock and human health, either directly or by acting as vectors of disease. The Sterile Insect Technique (SIT) - mass-release of sterile insects to mate with, and thereby control, their wild counterparts - has been used successfully for decades to control several pest species, including pink bollworm, a lepidopteran pest of cotton. Although it has been suggested that genetic engineering of pest insects provides potential improvements, there is uncertainty regarding its impact on their field performance. Discrimination between released and wild moths caught in monitoring traps is essential for estimating wild population levels. To address concerns about the reliability of current marking methods, we developed a genetically engineered strain of pink bollworm with a heritable fluorescent marker, to improve discrimination of sterile from wild moths. Here, we report the results of field trials showing that this engineered strain performed well under field conditions. Our data show that attributes critical to SIT in the field - ability to find a mate and to initiate copulation, as well as dispersal and persistence in the release area - were comparable between the genetically engineered strain and a standard strain. To our knowledge, these represent the first open-field experiments with a genetically engineered insect. The results described here provide encouragement for the genetic control of insect pests.

Original languageEnglish (US)
Article numbere24110
JournalPLoS One
Volume6
Issue number9
DOIs
StatePublished - Sep 13 2011

Fingerprint

genetically engineered microorganisms
Pectinophora gossypiella
sterile insect technique
insect pests
Insects
moths
Pest Control
disease vectors
Moths
genetic resistance
Pest control
genetic engineering
pest control
Genetic engineering
copulation
human health
field experimentation
uncertainty
cotton
livestock

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Simmons, G. S., McKemey, A. R., Morrison, N. I., O'Connell, S., Tabashnik, B. E., Claus, J., ... Alphey, L. (2011). Field performance of a genetically engineered strain of pink bollworm. PLoS One, 6(9), [e24110]. https://doi.org/10.1371/journal.pone.0024110

Field performance of a genetically engineered strain of pink bollworm. / Simmons, Gregory S.; McKemey, Andrew R.; Morrison, Neil I.; O'Connell, Sinead; Tabashnik, Bruce E; Claus, John; Fu, Guoliang; Tang, Guolei; Sledge, Mickey; Walker, Adam S.; Phillips, Caroline E.; Miller, Ernie D.; Rose, Robert I.; Staten, Robert T.; Donnelly, Christl A.; Alphey, Luke.

In: PLoS One, Vol. 6, No. 9, e24110, 13.09.2011.

Research output: Contribution to journalArticle

Simmons, GS, McKemey, AR, Morrison, NI, O'Connell, S, Tabashnik, BE, Claus, J, Fu, G, Tang, G, Sledge, M, Walker, AS, Phillips, CE, Miller, ED, Rose, RI, Staten, RT, Donnelly, CA & Alphey, L 2011, 'Field performance of a genetically engineered strain of pink bollworm', PLoS One, vol. 6, no. 9, e24110. https://doi.org/10.1371/journal.pone.0024110
Simmons GS, McKemey AR, Morrison NI, O'Connell S, Tabashnik BE, Claus J et al. Field performance of a genetically engineered strain of pink bollworm. PLoS One. 2011 Sep 13;6(9). e24110. https://doi.org/10.1371/journal.pone.0024110
Simmons, Gregory S. ; McKemey, Andrew R. ; Morrison, Neil I. ; O'Connell, Sinead ; Tabashnik, Bruce E ; Claus, John ; Fu, Guoliang ; Tang, Guolei ; Sledge, Mickey ; Walker, Adam S. ; Phillips, Caroline E. ; Miller, Ernie D. ; Rose, Robert I. ; Staten, Robert T. ; Donnelly, Christl A. ; Alphey, Luke. / Field performance of a genetically engineered strain of pink bollworm. In: PLoS One. 2011 ; Vol. 6, No. 9.
@article{b4c4cdff8ad44eb79916379daa4aab04,
title = "Field performance of a genetically engineered strain of pink bollworm",
abstract = "Pest insects harm crops, livestock and human health, either directly or by acting as vectors of disease. The Sterile Insect Technique (SIT) - mass-release of sterile insects to mate with, and thereby control, their wild counterparts - has been used successfully for decades to control several pest species, including pink bollworm, a lepidopteran pest of cotton. Although it has been suggested that genetic engineering of pest insects provides potential improvements, there is uncertainty regarding its impact on their field performance. Discrimination between released and wild moths caught in monitoring traps is essential for estimating wild population levels. To address concerns about the reliability of current marking methods, we developed a genetically engineered strain of pink bollworm with a heritable fluorescent marker, to improve discrimination of sterile from wild moths. Here, we report the results of field trials showing that this engineered strain performed well under field conditions. Our data show that attributes critical to SIT in the field - ability to find a mate and to initiate copulation, as well as dispersal and persistence in the release area - were comparable between the genetically engineered strain and a standard strain. To our knowledge, these represent the first open-field experiments with a genetically engineered insect. The results described here provide encouragement for the genetic control of insect pests.",
author = "Simmons, {Gregory S.} and McKemey, {Andrew R.} and Morrison, {Neil I.} and Sinead O'Connell and Tabashnik, {Bruce E} and John Claus and Guoliang Fu and Guolei Tang and Mickey Sledge and Walker, {Adam S.} and Phillips, {Caroline E.} and Miller, {Ernie D.} and Rose, {Robert I.} and Staten, {Robert T.} and Donnelly, {Christl A.} and Luke Alphey",
year = "2011",
month = "9",
day = "13",
doi = "10.1371/journal.pone.0024110",
language = "English (US)",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Field performance of a genetically engineered strain of pink bollworm

AU - Simmons, Gregory S.

AU - McKemey, Andrew R.

AU - Morrison, Neil I.

AU - O'Connell, Sinead

AU - Tabashnik, Bruce E

AU - Claus, John

AU - Fu, Guoliang

AU - Tang, Guolei

AU - Sledge, Mickey

AU - Walker, Adam S.

AU - Phillips, Caroline E.

AU - Miller, Ernie D.

AU - Rose, Robert I.

AU - Staten, Robert T.

AU - Donnelly, Christl A.

AU - Alphey, Luke

PY - 2011/9/13

Y1 - 2011/9/13

N2 - Pest insects harm crops, livestock and human health, either directly or by acting as vectors of disease. The Sterile Insect Technique (SIT) - mass-release of sterile insects to mate with, and thereby control, their wild counterparts - has been used successfully for decades to control several pest species, including pink bollworm, a lepidopteran pest of cotton. Although it has been suggested that genetic engineering of pest insects provides potential improvements, there is uncertainty regarding its impact on their field performance. Discrimination between released and wild moths caught in monitoring traps is essential for estimating wild population levels. To address concerns about the reliability of current marking methods, we developed a genetically engineered strain of pink bollworm with a heritable fluorescent marker, to improve discrimination of sterile from wild moths. Here, we report the results of field trials showing that this engineered strain performed well under field conditions. Our data show that attributes critical to SIT in the field - ability to find a mate and to initiate copulation, as well as dispersal and persistence in the release area - were comparable between the genetically engineered strain and a standard strain. To our knowledge, these represent the first open-field experiments with a genetically engineered insect. The results described here provide encouragement for the genetic control of insect pests.

AB - Pest insects harm crops, livestock and human health, either directly or by acting as vectors of disease. The Sterile Insect Technique (SIT) - mass-release of sterile insects to mate with, and thereby control, their wild counterparts - has been used successfully for decades to control several pest species, including pink bollworm, a lepidopteran pest of cotton. Although it has been suggested that genetic engineering of pest insects provides potential improvements, there is uncertainty regarding its impact on their field performance. Discrimination between released and wild moths caught in monitoring traps is essential for estimating wild population levels. To address concerns about the reliability of current marking methods, we developed a genetically engineered strain of pink bollworm with a heritable fluorescent marker, to improve discrimination of sterile from wild moths. Here, we report the results of field trials showing that this engineered strain performed well under field conditions. Our data show that attributes critical to SIT in the field - ability to find a mate and to initiate copulation, as well as dispersal and persistence in the release area - were comparable between the genetically engineered strain and a standard strain. To our knowledge, these represent the first open-field experiments with a genetically engineered insect. The results described here provide encouragement for the genetic control of insect pests.

UR - http://www.scopus.com/inward/record.url?scp=80052713070&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052713070&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0024110

DO - 10.1371/journal.pone.0024110

M3 - Article

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e24110

ER -