Fine structure of the band 3 protein in human red cell membranes

Freeze-fracture studies

Ronald S Weinstein, J. K. Khodadad, T. L. Steck

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The major red cell membrane protein, band 3, is a glycoprotein which extends across the membrane from the extracellular space into the cytoplasmic compartment. It is widely held that band 3 is a component of the intramembrane particles (IMP) which can be demonstrated by freeze-fracture electron microscopy. In this study, we find that the outer surface poles of the IMP can be seen by freeze-etching after they are unmasked by proteolysis under conditions which excise the surrounding sialopeptides from the membrane. The poles appear as distinctive projections, 30-50 Å in diameter, the 'ES particles.' The ES particles remain associated with the outer surface of the membrane following cleavage of the band 3 polypeptide by chymotrypsin or pronase. This is consistent with previous biochemical studies which have shown that the 38,000-dalton outer surface segment of band 3 is intercalated in the lipid bilayer. A granulofibrillar component at the inner surface of the membrane is provisionally identified as the 40,000-dalton inner-surface domain of band 3.

Original languageEnglish (US)
Title of host publicationProgress in Clinical and Biological Research
Pages47-57
Number of pages11
VolumeNo 30
StatePublished - 1979
Externally publishedYes

Fingerprint

Erythrocyte Anion Exchange Protein 1
Cell Membrane
Membranes
Freeze Etching
Pronase
Chymotrypsin
Extracellular Space
Lipid Bilayers
Proteolysis
Electron Microscopy
Glycoproteins
Membrane Proteins
Peptides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Weinstein, R. S., Khodadad, J. K., & Steck, T. L. (1979). Fine structure of the band 3 protein in human red cell membranes: Freeze-fracture studies. In Progress in Clinical and Biological Research (Vol. No 30, pp. 47-57)

Fine structure of the band 3 protein in human red cell membranes : Freeze-fracture studies. / Weinstein, Ronald S; Khodadad, J. K.; Steck, T. L.

Progress in Clinical and Biological Research. Vol. No 30 1979. p. 47-57.

Research output: Chapter in Book/Report/Conference proceedingChapter

Weinstein, RS, Khodadad, JK & Steck, TL 1979, Fine structure of the band 3 protein in human red cell membranes: Freeze-fracture studies. in Progress in Clinical and Biological Research. vol. No 30, pp. 47-57.
Weinstein RS, Khodadad JK, Steck TL. Fine structure of the band 3 protein in human red cell membranes: Freeze-fracture studies. In Progress in Clinical and Biological Research. Vol. No 30. 1979. p. 47-57
Weinstein, Ronald S ; Khodadad, J. K. ; Steck, T. L. / Fine structure of the band 3 protein in human red cell membranes : Freeze-fracture studies. Progress in Clinical and Biological Research. Vol. No 30 1979. pp. 47-57
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