FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor

Martha I. Gómez, M. Nurul Islam-Faridi, Sung Sick Woo, Keith F. Schertz, Don Czeschin, Michael S. Zwick, Rod A. Wing, David M. Stelly, H. James Price

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Fluorescence in situ hybridization (FISH) of a 205 kb Sorghum bicolor bacterial artificial chromosome (BAC) containing a sequence complementary to maize sh2 cDNA produced a large pair of FISH signals at one end of a midsize metacentric chromosome of S. bicolor. Three pairs of signals were observed in metaphase spreads of chromosomes of a sorghum plant containing an extra copy of one arm of the sorghum chromosome arbitrarily designated with the letter D. Therefore, the sequence cloned in this BAC must reside in the arm of chromosome D represented by this monotelosome. This demonstrates a novel procedure for physically mapping cloned genes or other single-copy sequences by FISH, sh2 in this case, by using BACs containing their complementary sequences. The results reported herein suggest homology, at least in part, between one arm of chromosome D in sorghum and the long arm of chromosome 3 in maize.

Original languageEnglish (US)
Pages (from-to)475-478
Number of pages4
JournalGenome
Volume40
Issue number4
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • Bacterial artificial chromosomes
  • Fluorescence in situ hybridization
  • Maize
  • Physical mapping
  • Shrunken locus
  • Sorghum

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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    Gómez, M. I., Islam-Faridi, M. N., Woo, S. S., Schertz, K. F., Czeschin, D., Zwick, M. S., Wing, R. A., Stelly, D. M., & Price, H. J. (1997). FISH of a maize sh2-selected sorghum BAC to chromosomes of Sorghum bicolor. Genome, 40(4), 475-478. https://doi.org/10.1139/g97-063