Flow-rate dependent capture of circulating tumor cells in bio-functional microchannels

Xiangjun Zheng, Linan Jiang, Joyce A. Schroeder, Marilyn Marron, Maria Iannone, Alison T. Stopeck, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The capture of circulating tumor cells (CTCs) in shear flow is studied systematically by driving suspensions of breast cancer cells, with different EpCAM receptor expression levels, through microchannels functionalized with EpCAM antibody ligands at various densities. The dependence of cell capture efficiency on flow rate is established and can be described by an exponential function; a characteristic flow rate immerges as the most dominant parameter affecting the capture efficiency. Utilizing this characteristic flow rate as a scaling factor, all measured capture efficiencies for the tested receptor and ligand densities can be expressed using a single exponential formula. The effect of both ligand and receptor densities on the characteristic flow rate is found to be a product of two independent cumulative distributions.

Original languageEnglish (US)
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages82-86
Number of pages5
DOIs
StatePublished - 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: Nov 4 2012Nov 7 2012

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period11/4/1211/7/12

Keywords

  • Cell capture
  • Cell-receptor density
  • Circulating tumor cells
  • Microfluidics
  • Surface-ligand density

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

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  • Cite this

    Zheng, X., Jiang, L., Schroeder, J. A., Marron, M., Iannone, M., Stopeck, A. T., & Zohar, Y. (2012). Flow-rate dependent capture of circulating tumor cells in bio-functional microchannels. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 82-86). [6509114] (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509114