Flow sorting and culture of protoplasts: Conditions for high-frequency recovery, growth and morphogenesis from sorted protoplasts of suspension cultures of nicotiana

D. W. Galbraith, C. L. Afonso, K. R. Harkins

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Protoplasts were prepared from suspension cultures of Nicotiana tabacum cv Wisconsin 38 that had been prelabeled with FITC. The protoplasts were subjected to flow sorting based on fluorescence content using a Coulter EPICS V Flow Cytometer - Cell Sorter. Conditions were established that allowed the recovery after sorting of approximately 30% of the initial protoplasts in a viable state. These were subsequently regenerated into calli that underwent shoot morphogenesis.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalPlant Cell Reports
Volume3
Issue number4
DOIs
StatePublished - Aug 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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