FLP-mediated site-specific recombination in microinjected murine zygotes

Dale L. Ludwig, James R. Stringer, David C. Wight, Thomas C Doetschman, John J. Duffy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The FLP recombinase of yeast catalyses site-specific recombination between repeated FLP recombinase target (FRT) elements in yeast and in heterologous systems (Escherichia coli, Drosophila, mosquito and cultured mammalian cells). In this report, it is shown that transient FLP recombinase expression can recombine and activate an extrachromosomal silent reporter gene following coinjection into fertilized one-cell mouse eggs. Furthermore, it is demonstrated that introduction of a FLP-recombinase expression vector into transgenic one-cell fertilized mouse eggs induces a recombination event at a chromosomal FRT target locus. The resulting event occured at the one- cell stage and deleted a chromosomal tandem array of a FRT containing lacZ expression cassette down to one or two copies. These results demonstrate that the FLP recombinase can be utilized to manipulate the genome of transgenic animals and suggest that FLP recombinase-mediated plasmid-to-chromosome targeting is feasible in microinjected eggs.

Original languageEnglish (US)
Pages (from-to)385-395
Number of pages11
JournalTransgenic Research
Volume5
Issue number6
StatePublished - Nov 1996
Externally publishedYes

Fingerprint

site-specific recombination
Zygote
zygote
Genetic Recombination
mice
cells
yeasts
transgenic animals
reporter genes
Eggs
Drosophila
plasmids
Culicidae
Yeasts
genetically modified organisms
Escherichia coli
chromosomes
loci
Genetically Modified Animals
genome

Keywords

  • gene targeting
  • lacZ
  • recombinase
  • transgenic

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

Ludwig, D. L., Stringer, J. R., Wight, D. C., Doetschman, T. C., & Duffy, J. J. (1996). FLP-mediated site-specific recombination in microinjected murine zygotes. Transgenic Research, 5(6), 385-395.

FLP-mediated site-specific recombination in microinjected murine zygotes. / Ludwig, Dale L.; Stringer, James R.; Wight, David C.; Doetschman, Thomas C; Duffy, John J.

In: Transgenic Research, Vol. 5, No. 6, 11.1996, p. 385-395.

Research output: Contribution to journalArticle

Ludwig, DL, Stringer, JR, Wight, DC, Doetschman, TC & Duffy, JJ 1996, 'FLP-mediated site-specific recombination in microinjected murine zygotes', Transgenic Research, vol. 5, no. 6, pp. 385-395.
Ludwig, Dale L. ; Stringer, James R. ; Wight, David C. ; Doetschman, Thomas C ; Duffy, John J. / FLP-mediated site-specific recombination in microinjected murine zygotes. In: Transgenic Research. 1996 ; Vol. 5, No. 6. pp. 385-395.
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