FLP-mediated site-specific recombination in microinjected murine zygotes

Dale L. Ludwig, James R. Stringer, David C. Wight, Thomas C. Doetschman, John J. Duffy

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The FLP recombinase of yeast catalyses site-specific recombination between repeated FLP recombinase target (FRT) elements in yeast and in heterologous systems (Escherichia coli, Drosophila, mosquito and cultured mammalian cells). In this report, it is shown that transient FLP recombinase expression can recombine and activate an extrachromosomal silent reporter gene following coinjection into fertilized one-cell mouse eggs. Furthermore, it is demonstrated that introduction of a FLP-recombinase expression vector into transgenic one-cell fertilized mouse eggs induces a recombination event at a chromosomal FRT target locus. The resulting event occured at the one- cell stage and deleted a chromosomal tandem array of a FRT containing lacZ expression cassette down to one or two copies. These results demonstrate that the FLP recombinase can be utilized to manipulate the genome of transgenic animals and suggest that FLP recombinase-mediated plasmid-to-chromosome targeting is feasible in microinjected eggs.

Original languageEnglish (US)
Pages (from-to)385-395
Number of pages11
JournalTransgenic Research
Volume5
Issue number6
DOIs
StatePublished - 1996

Keywords

  • gene targeting
  • lacZ
  • recombinase
  • transgenic

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

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