Fluid absorption with and without sodium in isolated perfused snake proximal tubules

William H Dantzler, S. K. Bentley

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Citations (Scopus)

Abstract

Net fluid absorption (Jv) was studied in isolated, perfused snake (Thamnophis spp.) proximal renal tubules. With standard (150 mmol/liter Na+) bicarbonate-buffered Ringer in perfusate and standard Ringer plus dextran (4 g/100 ml) in bath, Jv was about 0.9 nl min-1 mm-1. Removing dextran from bath reduced Jv by about 20%. When sodium in perfusate was replaced with choline. Jv approached zero. However, when sodium in bath as well as perfusate was replaced with choline, Jv returned to control level. Results were the same when sodium was replaced with tetramethylammonium, sodium chloride was replaced with sucrose or lactose, or chloride was replaced with methyl sulfate. In contrast, replacing sodium in perfusate or in both perfusate and bath with lithium did not reduce Jv. Fluid absorption was always isosmotic. Replacing bicarbonate with phosphate or Tris in sodium-containing media had no effect on Jv, but the presence of buffer in sodium-free or low-chloride media may have been important for Jv. Reducing temperature 10°C reduced Jv by about 35% with either sodium chloride or sucrose in both perfusate and bath. The results indicate that isosmotic fluid absoprtion can occur when lithium is substituted for sodium or when some other substitution is made for sodium, chloride, or both in perfusate and bath simultaneously.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume3
Edition1
StatePublished - 1978

Fingerprint

Snakes
Baths
Sodium
Sodium Chloride
Bicarbonates
Choline
Dextrans
Lithium
Sucrose
Chlorides
Colubridae
Proximal Kidney Tubule
Lactose
Buffers
Phosphates
Temperature

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Dantzler, W. H., & Bentley, S. K. (1978). Fluid absorption with and without sodium in isolated perfused snake proximal tubules. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (1 ed., Vol. 3)

Fluid absorption with and without sodium in isolated perfused snake proximal tubules. / Dantzler, William H; Bentley, S. K.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 3 1. ed. 1978.

Research output: Chapter in Book/Report/Conference proceedingChapter

Dantzler, WH & Bentley, SK 1978, Fluid absorption with and without sodium in isolated perfused snake proximal tubules. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1 edn, vol. 3.
Dantzler WH, Bentley SK. Fluid absorption with and without sodium in isolated perfused snake proximal tubules. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1 ed. Vol. 3. 1978
Dantzler, William H ; Bentley, S. K. / Fluid absorption with and without sodium in isolated perfused snake proximal tubules. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 3 1. ed. 1978.
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