Fluid absorption with and without sodium in isolated perfused snake proximal tubules.

William H Dantzler, S. K. Bentley

Research output: Contribution to journalArticle

Abstract

Net fluid absorption (JV) was studied in isolated, perfused snake (Thamnophis spp.) proximal renal tubules. With standard (150 mmol/liter Na+) bicarbonate-buffered Ringer in perfusate and standard Ringer plus dextran (4 g/100 ml) in bath, JV was about 0.9 nl min-1 mm-1. Removing dextran from bath reduced JV by about 20 percent. When sodium in perfusate was replaced with choline JV approached zero. However, when sodium in bath as well as perfusate was replaced with choline, JV returned to control level. Results were the same when sodium was replaced with tetramethyl-ammonium, sodium chloride was replaced with sucrose or lactose, or chloride was replaced with methyl sulfate. In contrast, replacing sodium in perfusate or in both perfusate and bath with lithium did not reduce JV. Fluid absorption was always isosmotic. Replacing bicarbonate with phosphate or Tris in sodium-containing media had no effect on JV, but the presence of buffer in sodium-free or low-chloride media may have been important for JV. Reducing temperature 10 degrees C reduced JV by about 35 percent with either sodium chloride or sucrose in both perfusate and bath. The results indicate that isosmotic fluid absorption can occur when lithium is substituted for sodium or when some other substitution is made for sodium, chloride, or both in perfusate and bath simultaneously.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume234
Issue number1
StatePublished - Jan 1978
Externally publishedYes

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Snakes
Baths
Sodium
Sodium Chloride
Bicarbonates
Choline
Dextrans
Lithium
Sucrose
Chlorides
Colubridae
Ammonium Chloride
Proximal Kidney Tubule
Lactose
Buffers
Phosphates
Temperature

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fluid absorption with and without sodium in isolated perfused snake proximal tubules. / Dantzler, William H; Bentley, S. K.

In: The American journal of physiology, Vol. 234, No. 1, 01.1978.

Research output: Contribution to journalArticle

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