Fluorescence-activated cell sorting of embryonic mouse and rat motoneurons and their long-term survival in vitro.

A. E. Schaffner, Paul A St John, J. L. Barker

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Abstract

Motoneurons from embryonic mice and rats were labeled with retrogradely transported succinyl wheat germ agglutinin (WGA)-fluorescein isothiocyanate (FITC). After dissociation of the spinal cord, fluorescent motoneurons were isolated by flow cytometry. Sorted motoneurons were maintained for as long as 6 weeks in vitro on monolayers of astrocytes in muscle-conditioned medium. Immunocytochemical staining of the cultures for various neuronal antigens suggested that sorted motoneurons are receptive to GABAergic and glycinergic, as well as cholinergic, innervation. Many of the sorted cells were also labeled intracellularly with antibodies to choline acetyltransferase (ChAT) and GABA.

Original languageEnglish (US)
Pages (from-to)3088-3104
Number of pages17
JournalJournal of Neuroscience
Volume7
Issue number10
Publication statusPublished - Oct 1987
Externally publishedYes

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ASJC Scopus subject areas

  • Neuroscience(all)

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