Fluorescence-activated cell sorting of embryonic mouse and rat motoneurons and their long-term survival in vitro.

A. E. Schaffner, P. A. St John, J. L. Barker

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Motoneurons from embryonic mice and rats were labeled with retrogradely transported succinyl wheat germ agglutinin (WGA)-fluorescein isothiocyanate (FITC). After dissociation of the spinal cord, fluorescent motoneurons were isolated by flow cytometry. Sorted motoneurons were maintained for as long as 6 weeks in vitro on monolayers of astrocytes in muscle-conditioned medium. Immunocytochemical staining of the cultures for various neuronal antigens suggested that sorted motoneurons are receptive to GABAergic and glycinergic, as well as cholinergic, innervation. Many of the sorted cells were also labeled intracellularly with antibodies to choline acetyltransferase (ChAT) and GABA.

Original languageEnglish (US)
Pages (from-to)3088-3104
Number of pages17
JournalThe Journal of neuroscience : the official journal of the Society for Neuroscience
Volume7
Issue number10
DOIs
StatePublished - Oct 1987

ASJC Scopus subject areas

  • Neuroscience(all)

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