Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis

Ioanna Antoniadi, Vladimír Skalický, Guiling Sun, Wen Ma, David W. Galbraith, Ondřej Novák, Karin Ljung

Research output: Contribution to journalReview articlepeer-review

Abstract

Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.

Original languageEnglish (US)
JournalCytometry Part A
DOIs
StateAccepted/In press - 2021

Keywords

  • autofluorescence
  • best practices
  • plant flow cytometry and sorting
  • protoplasts
  • viability and integrity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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