Fluorescence and reflectance spectral imaging system for a murine mammary window chamber model

Hui Min Leung, Arthur F Gmitro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A spectral imaging system was developed to study the development of breast cancer xenografts in a murine mammary window chamber model. The instrument is configured to work with either a laser to excite fluorescence or a broadband light source for diffuse reflectance imaging. Two applications were demonstrated. First, spectral imaging of fluorescence signals was demonstrated with a GFP-breast cancer tumor and fluorescein injection. Second, based on the principles of broadband reflectance spectroscopy, the instrument was used to monitor dynamic changes of tissue absorbance to yield tissue oxygenation maps at different time points during tumor progression.

Original languageEnglish (US)
Article numberA002
Pages (from-to)2887-2894
Number of pages8
JournalBiomedical Optics Express
Volume6
Issue number8
DOIs
StatePublished - 2015

Fingerprint

spectral reflectance
breast
Breast
tumors
chambers
Fluorescence
cancer
Breast Neoplasms
broadband
reflectance
fluorescence
oxygenation
progressions
light sources
Optical Imaging
injection
Fluorescein
Heterografts
Spectrum Analysis
Lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Fluorescence and reflectance spectral imaging system for a murine mammary window chamber model. / Leung, Hui Min; Gmitro, Arthur F.

In: Biomedical Optics Express, Vol. 6, No. 8, A002, 2015, p. 2887-2894.

Research output: Contribution to journalArticle

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