Fluorescence bleach rate imaging

G. J. BRAKENHOFF, K. VISSCHER, E. J. GIJSBERS

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Bleach rate imaging on a (cooled) CCD can be easily achieved using a confocal microscope with bilateral scanning and detection coupled to a workstation; it is as easy as acquiring regular fluorescence images. Several analysis and display methods for bleach rate imaging are presented such as the bleach map (and its inverse) and a matrix‐based decomposition method for multi‐labelled specimens based on the bleach rate differences between the dyes used. With these tools, bleach‐rate‐based imaging can become a viable alternative to multiple labelling techniques for component identification in fluorescent specimens. 1994 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalJournal of Microscopy
Volume175
Issue number2
DOIs
StatePublished - Aug 1994
Externally publishedYes

Keywords

  • CCD
  • Fluorescence microscopy
  • bilateral
  • bleach map
  • bleach rate
  • confocal
  • histomap
  • photobleaching
  • photodegradation
  • ratio‐imaging

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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