Fluorescence bleach rate imaging

G. J. Brakenhoff, Koen Visscher, E. J. Gijsbers

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Bleach rate imaging on a (cooled) CCD can be easily achieved using a confocal microscope with bilateral scanning and detection coupled to a workstation; it is as easy as acquiring regular fluorescence images. Several analysis and display methods for bleach rate imaging are presented such as the bleach map (and its inverse) and a matrix-based decomposition method for multi-labelled specimens based on the bleach rate differences between the dyes used. With these tools, bleach-rate-based imaging can become a viable alternative to multiple labelling techniques for component identification in fluorescent specimens.

Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalJournal of Microscopy
Volume175
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Fluorescence
Imaging techniques
fluorescence
Coloring Agents
Charge coupled devices
Labeling
workstations
Microscopes
Dyes
Display devices
marking
Decomposition
Scanning
charge coupled devices
dyes
microscopes
decomposition
scanning
matrices

Keywords

  • bilateral
  • bleach map
  • bleach rate
  • CCD
  • confocal
  • Fluorescence microscopy
  • histomap
  • photobleaching
  • photodegradation
  • ratio-imaging

ASJC Scopus subject areas

  • Instrumentation

Cite this

Brakenhoff, G. J., Visscher, K., & Gijsbers, E. J. (1994). Fluorescence bleach rate imaging. Journal of Microscopy, 175(2), 154-161.

Fluorescence bleach rate imaging. / Brakenhoff, G. J.; Visscher, Koen; Gijsbers, E. J.

In: Journal of Microscopy, Vol. 175, No. 2, 1994, p. 154-161.

Research output: Contribution to journalArticle

Brakenhoff, GJ, Visscher, K & Gijsbers, EJ 1994, 'Fluorescence bleach rate imaging', Journal of Microscopy, vol. 175, no. 2, pp. 154-161.
Brakenhoff GJ, Visscher K, Gijsbers EJ. Fluorescence bleach rate imaging. Journal of Microscopy. 1994;175(2):154-161.
Brakenhoff, G. J. ; Visscher, Koen ; Gijsbers, E. J. / Fluorescence bleach rate imaging. In: Journal of Microscopy. 1994 ; Vol. 175, No. 2. pp. 154-161.
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