Fluorescence excitation emission matrices of human tissue: a system for in vivo measurement and method of data analysis

Andrés F. Zuluaga, Urs Utzinger, Anthony Durkin, Holger Fuchs, Ann Gillenwater, Rhonda Jacob, Bonnie Kemp, James Fan, Rebecca Richards-Kortum

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This 'FastEEM' system was used to interrogate human normal and neoplastic oral cavity mucosa in vivo. Measurements were made through a fiber-optic probe and require 4 min total measurement time. We present a method based on autocorrelation vectors to identify excitation and emission wavelengths where the spectra of normal and pathologic tissues differ most. The FastEEM system provides a tool with which to study the relative diagnostic ability of changes in absorption, scattering, and fluorescence properties of tissue.

Original languageEnglish (US)
Pages (from-to)302-311
Number of pages10
JournalApplied Spectroscopy
Volume53
Issue number3
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

Fluorescence
Tissue
fluorescence
matrices
Time measurement
Autocorrelation
Fiber optics
excitation
Scattering
Scanning
Wavelength
autocorrelation
fiber optics
time measurement
reflectance
cavities
scanning
probes
scattering
wavelengths

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Zuluaga, A. F., Utzinger, U., Durkin, A., Fuchs, H., Gillenwater, A., Jacob, R., ... Richards-Kortum, R. (1999). Fluorescence excitation emission matrices of human tissue: a system for in vivo measurement and method of data analysis. Applied Spectroscopy, 53(3), 302-311.

Fluorescence excitation emission matrices of human tissue : a system for in vivo measurement and method of data analysis. / Zuluaga, Andrés F.; Utzinger, Urs; Durkin, Anthony; Fuchs, Holger; Gillenwater, Ann; Jacob, Rhonda; Kemp, Bonnie; Fan, James; Richards-Kortum, Rebecca.

In: Applied Spectroscopy, Vol. 53, No. 3, 03.1999, p. 302-311.

Research output: Contribution to journalArticle

Zuluaga, AF, Utzinger, U, Durkin, A, Fuchs, H, Gillenwater, A, Jacob, R, Kemp, B, Fan, J & Richards-Kortum, R 1999, 'Fluorescence excitation emission matrices of human tissue: a system for in vivo measurement and method of data analysis', Applied Spectroscopy, vol. 53, no. 3, pp. 302-311.
Zuluaga, Andrés F. ; Utzinger, Urs ; Durkin, Anthony ; Fuchs, Holger ; Gillenwater, Ann ; Jacob, Rhonda ; Kemp, Bonnie ; Fan, James ; Richards-Kortum, Rebecca. / Fluorescence excitation emission matrices of human tissue : a system for in vivo measurement and method of data analysis. In: Applied Spectroscopy. 1999 ; Vol. 53, No. 3. pp. 302-311.
@article{3e6111205f744e69972886a3df9c9aa2,
title = "Fluorescence excitation emission matrices of human tissue: a system for in vivo measurement and method of data analysis",
abstract = "We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This 'FastEEM' system was used to interrogate human normal and neoplastic oral cavity mucosa in vivo. Measurements were made through a fiber-optic probe and require 4 min total measurement time. We present a method based on autocorrelation vectors to identify excitation and emission wavelengths where the spectra of normal and pathologic tissues differ most. The FastEEM system provides a tool with which to study the relative diagnostic ability of changes in absorption, scattering, and fluorescence properties of tissue.",
author = "Zuluaga, {Andr{\'e}s F.} and Urs Utzinger and Anthony Durkin and Holger Fuchs and Ann Gillenwater and Rhonda Jacob and Bonnie Kemp and James Fan and Rebecca Richards-Kortum",
year = "1999",
month = "3",
language = "English (US)",
volume = "53",
pages = "302--311",
journal = "Applied Spectroscopy",
issn = "0003-7028",
publisher = "Society for Applied Spectroscopy",
number = "3",

}

TY - JOUR

T1 - Fluorescence excitation emission matrices of human tissue

T2 - a system for in vivo measurement and method of data analysis

AU - Zuluaga, Andrés F.

AU - Utzinger, Urs

AU - Durkin, Anthony

AU - Fuchs, Holger

AU - Gillenwater, Ann

AU - Jacob, Rhonda

AU - Kemp, Bonnie

AU - Fan, James

AU - Richards-Kortum, Rebecca

PY - 1999/3

Y1 - 1999/3

N2 - We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This 'FastEEM' system was used to interrogate human normal and neoplastic oral cavity mucosa in vivo. Measurements were made through a fiber-optic probe and require 4 min total measurement time. We present a method based on autocorrelation vectors to identify excitation and emission wavelengths where the spectra of normal and pathologic tissues differ most. The FastEEM system provides a tool with which to study the relative diagnostic ability of changes in absorption, scattering, and fluorescence properties of tissue.

AB - We describe a system capable of measuring spatially resolved reflectance spectra from 380 to 950 nm and fluorescence excitation emission matrices from 330 to 500 nm excitation and 380 to 700 nm emission in vivo. System performance was compared to that of a standard scanning spectrofluorimeter. This 'FastEEM' system was used to interrogate human normal and neoplastic oral cavity mucosa in vivo. Measurements were made through a fiber-optic probe and require 4 min total measurement time. We present a method based on autocorrelation vectors to identify excitation and emission wavelengths where the spectra of normal and pathologic tissues differ most. The FastEEM system provides a tool with which to study the relative diagnostic ability of changes in absorption, scattering, and fluorescence properties of tissue.

UR - http://www.scopus.com/inward/record.url?scp=0032633518&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032633518&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032633518

VL - 53

SP - 302

EP - 311

JO - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 0003-7028

IS - 3

ER -