Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma

M. Lu-Steffes, G. W. Pittluck, M. E. Jolley, H. N. Panas, D. L. Olive, C. H. Wang, D. D. Nystrom, C. L. Keegan, Thomas P Davis, S. D. Stroupe

Research output: Contribution to journalArticle

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Abstract

Fluorescence polarization immunoassays for phenytoin and phenobarbital in human serum or plasma are described and shown to be clinically useful. No sample pretreatment, extraction, or phase separation is required. A determination can be made with less than 20 μL of sample in 15 min, with incubation at ambient temperature. Abnormal concentrations of protein, lipid, hemoglobin, or bilirubin do not interfere. In addition, drug metabolites and commonly co-administered drugs do not affect the assay results at clinically significant concentrations. Analytical recoveries of each of the two anticonvulsant drugs from serum averaged 101%. Between-assay CVs were <6.5%, with sensitivities of 0.5 mg/L. Comparison of this method with 'high-performance' liquid chromatography, homogeneous enzyme immunoassay, and radioimmunoassay for phenytoin determinations yielded correlations of 0.99, 0.98, and 0.99, respectively. Similar comparison studies for phenobarbital yielded correlation coefficients of 0.99, 0.98, and 0.99, respectively.

Original languageEnglish (US)
Pages (from-to)2278-2282
Number of pages5
JournalClinical Chemistry
Volume28
Issue number11
StatePublished - 1982
Externally publishedYes

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Fluorescence Polarization Immunoassay
Phenytoin
Phenobarbital
Assays
Fluorescence
Polarization
Plasmas
High performance liquid chromatography
Metabolites
Serum
Immunoenzyme Techniques
Bilirubin
Phase separation
Pharmaceutical Preparations
Anticonvulsants
Radioimmunoassay
Hemoglobins
High Pressure Liquid Chromatography
Lipids
Recovery

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Lu-Steffes, M., Pittluck, G. W., Jolley, M. E., Panas, H. N., Olive, D. L., Wang, C. H., ... Stroupe, S. D. (1982). Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma. Clinical Chemistry, 28(11), 2278-2282.

Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma. / Lu-Steffes, M.; Pittluck, G. W.; Jolley, M. E.; Panas, H. N.; Olive, D. L.; Wang, C. H.; Nystrom, D. D.; Keegan, C. L.; Davis, Thomas P; Stroupe, S. D.

In: Clinical Chemistry, Vol. 28, No. 11, 1982, p. 2278-2282.

Research output: Contribution to journalArticle

Lu-Steffes, M, Pittluck, GW, Jolley, ME, Panas, HN, Olive, DL, Wang, CH, Nystrom, DD, Keegan, CL, Davis, TP & Stroupe, SD 1982, 'Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma', Clinical Chemistry, vol. 28, no. 11, pp. 2278-2282.
Lu-Steffes M, Pittluck GW, Jolley ME, Panas HN, Olive DL, Wang CH et al. Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma. Clinical Chemistry. 1982;28(11):2278-2282.
Lu-Steffes, M. ; Pittluck, G. W. ; Jolley, M. E. ; Panas, H. N. ; Olive, D. L. ; Wang, C. H. ; Nystrom, D. D. ; Keegan, C. L. ; Davis, Thomas P ; Stroupe, S. D. / Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma. In: Clinical Chemistry. 1982 ; Vol. 28, No. 11. pp. 2278-2282.
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