Fluorescence quenching high-performance thin-layer chromatographic analysis utilizing a scientifically operated charge-coupled device detector

R. E. Simon, L. K. Walton, Y. Liang, M Bonner Denton

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A scientifically operated charge-coupled device detector combined with fluorescence quenching high-performance thin-layer chromatographic plates was employed for the detection of organic compounds. The plates were excited with 254 nm light from a mercury lamp, and quantitative information was obtained from organic compounds that absorbed the optimum conditions for detection. The linear dynamic range, sensitivity, and reproducibility of the system were evaluted by quantitative analysis of famotidine, acetaminophen, caffeine, and acetylsalicylic acid. The detection limits of the system were found to be in the nanogram range.

Original languageEnglish (US)
Pages (from-to)446-450
Number of pages5
JournalAnalyst
Volume126
Issue number4
DOIs
StatePublished - 2001

Fingerprint

Famotidine
Thin layer chromatography
Acetaminophen
Thin Layer Chromatography
Caffeine
Mercury
Organic compounds
Charge coupled devices
Aspirin
Limit of Detection
Quenching
fluorescence
Fluorescence
Detectors
Mercury vapor lamps
Light
Equipment and Supplies
organic compound
quantitative analysis
Acids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Fluorescence quenching high-performance thin-layer chromatographic analysis utilizing a scientifically operated charge-coupled device detector. / Simon, R. E.; Walton, L. K.; Liang, Y.; Denton, M Bonner.

In: Analyst, Vol. 126, No. 4, 2001, p. 446-450.

Research output: Contribution to journalArticle

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