Fluorescence quenching high-performance thin-layer chromatographic analysis utilizing a scientifically operated charge-coupled device detector

R. E. Simon, L. K. Walton, Y. Liang, M. B. Denton

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A scientifically operated charge-coupled device detector combined with fluorescence quenching high-performance thin-layer chromatographic plates was employed for the detection of organic compounds. The plates were excited with 254 nm light from a mercury lamp, and quantitative information was obtained from organic compounds that absorbed the optimum conditions for detection. The linear dynamic range, sensitivity, and reproducibility of the system were evaluted by quantitative analysis of famotidine, acetaminophen, caffeine, and acetylsalicylic acid. The detection limits of the system were found to be in the nanogram range.

Original languageEnglish (US)
Pages (from-to)446-450
Number of pages5
JournalAnalyst
Volume126
Issue number4
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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