Fluorescence saturation in confocal microscopy

K. VISSCHER, G. J. BRAKENHOFF, T. D. VISSER

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The effects of fluorescence saturation on imaging in confocal microscopy have been studied. To include saturation it was necessary to deviate from the widely assumed linear relationship between the fluorescence and the illumination intensity. The lateral response for a point‐like object, as well as the optical sectioning power, decreases depending on the degree of saturation. For very high illumination intensities the response for a saturated point object approached that of a conventional fluorescence microscope in which the fluorescence was not saturated. The decrease in the axial confocal response has been confirmed qualitatively by experiment. 1994 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)162-165
Number of pages4
JournalJournal of Microscopy
Volume175
Issue number2
DOIs
StatePublished - Aug 1994
Externally publishedYes

Keywords

  • Fluorescence
  • confocal microscopy
  • saturation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Fingerprint Dive into the research topics of 'Fluorescence saturation in confocal microscopy'. Together they form a unique fingerprint.

  • Cite this