Fluorescence saturation in confocal microscopy

Koen Visscher, G. J. Brakenhoff, T. D. Visser

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effects of fluorescence saturation on imaging in confocal microscopy have been studied. To include saturation it was necessary to deviate from the widely assumed linear relationship between the fluorescence and the illumination intensity. The lateral response for a point-like object, as well as the optical sectioning power, decreases depending on the degree of saturation. For very high illumination intensities the response for a saturated point object approached that of a conventional fluorescence microscope in which the fluorescence was not saturated. The decrease in the axial confocal response has been confirmed qualitatively by experiment.

Original languageEnglish (US)
Pages (from-to)162-165
Number of pages4
JournalJournal of Microscopy
Volume175
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Confocal microscopy
Confocal Microscopy
Fluorescence
microscopy
saturation
fluorescence
Lighting
illumination
Microscopes
microscopes
Imaging techniques
Experiments

Keywords

  • confocal microscopy
  • Fluorescence
  • saturation

ASJC Scopus subject areas

  • Instrumentation

Cite this

Visscher, K., Brakenhoff, G. J., & Visser, T. D. (1994). Fluorescence saturation in confocal microscopy. Journal of Microscopy, 175(2), 162-165.

Fluorescence saturation in confocal microscopy. / Visscher, Koen; Brakenhoff, G. J.; Visser, T. D.

In: Journal of Microscopy, Vol. 175, No. 2, 1994, p. 162-165.

Research output: Contribution to journalArticle

Visscher, K, Brakenhoff, GJ & Visser, TD 1994, 'Fluorescence saturation in confocal microscopy', Journal of Microscopy, vol. 175, no. 2, pp. 162-165.
Visscher K, Brakenhoff GJ, Visser TD. Fluorescence saturation in confocal microscopy. Journal of Microscopy. 1994;175(2):162-165.
Visscher, Koen ; Brakenhoff, G. J. ; Visser, T. D. / Fluorescence saturation in confocal microscopy. In: Journal of Microscopy. 1994 ; Vol. 175, No. 2. pp. 162-165.
@article{c04acf8616af498891dba1be18df6cd2,
title = "Fluorescence saturation in confocal microscopy",
abstract = "The effects of fluorescence saturation on imaging in confocal microscopy have been studied. To include saturation it was necessary to deviate from the widely assumed linear relationship between the fluorescence and the illumination intensity. The lateral response for a point-like object, as well as the optical sectioning power, decreases depending on the degree of saturation. For very high illumination intensities the response for a saturated point object approached that of a conventional fluorescence microscope in which the fluorescence was not saturated. The decrease in the axial confocal response has been confirmed qualitatively by experiment.",
keywords = "confocal microscopy, Fluorescence, saturation",
author = "Koen Visscher and Brakenhoff, {G. J.} and Visser, {T. D.}",
year = "1994",
language = "English (US)",
volume = "175",
pages = "162--165",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Fluorescence saturation in confocal microscopy

AU - Visscher, Koen

AU - Brakenhoff, G. J.

AU - Visser, T. D.

PY - 1994

Y1 - 1994

N2 - The effects of fluorescence saturation on imaging in confocal microscopy have been studied. To include saturation it was necessary to deviate from the widely assumed linear relationship between the fluorescence and the illumination intensity. The lateral response for a point-like object, as well as the optical sectioning power, decreases depending on the degree of saturation. For very high illumination intensities the response for a saturated point object approached that of a conventional fluorescence microscope in which the fluorescence was not saturated. The decrease in the axial confocal response has been confirmed qualitatively by experiment.

AB - The effects of fluorescence saturation on imaging in confocal microscopy have been studied. To include saturation it was necessary to deviate from the widely assumed linear relationship between the fluorescence and the illumination intensity. The lateral response for a point-like object, as well as the optical sectioning power, decreases depending on the degree of saturation. For very high illumination intensities the response for a saturated point object approached that of a conventional fluorescence microscope in which the fluorescence was not saturated. The decrease in the axial confocal response has been confirmed qualitatively by experiment.

KW - confocal microscopy

KW - Fluorescence

KW - saturation

UR - http://www.scopus.com/inward/record.url?scp=0028133340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028133340&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0028133340

VL - 175

SP - 162

EP - 165

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - 2

ER -