Fluorescent and scattering contrast agents in a mouse model of colorectal cancer

Amy M. Winkler, Photini F S Rice, Timothy S. Troutman, Marina V. Backer, Joseph M. Backer, Rebekah A. Drezek, Marek Romanowski, Jennifer K Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6851
DOIs
StatePublished - 2008
EventEndoscopic Microscopy III - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Other

OtherEndoscopic Microscopy III
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

Fingerprint

Optical tomography
Scattering
Fluorescence
Lasers
Nanoshells
Wavelength
Fluorophores
Nanorods
Silver
Microscopes
Contamination
Dyes
Gold
Tissue
Imaging techniques

Keywords

  • Colon cancer
  • Laser-induced fluorescence
  • Optical coherence tomography

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Winkler, A. M., Rice, P. F. S., Troutman, T. S., Backer, M. V., Backer, J. M., Drezek, R. A., ... Barton, J. K. (2008). Fluorescent and scattering contrast agents in a mouse model of colorectal cancer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6851). [68510L] https://doi.org/10.1117/12.764043

Fluorescent and scattering contrast agents in a mouse model of colorectal cancer. / Winkler, Amy M.; Rice, Photini F S; Troutman, Timothy S.; Backer, Marina V.; Backer, Joseph M.; Drezek, Rebekah A.; Romanowski, Marek; Barton, Jennifer K.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6851 2008. 68510L.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Winkler, AM, Rice, PFS, Troutman, TS, Backer, MV, Backer, JM, Drezek, RA, Romanowski, M & Barton, JK 2008, Fluorescent and scattering contrast agents in a mouse model of colorectal cancer. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6851, 68510L, Endoscopic Microscopy III, San Jose, CA, United States, 1/20/08. https://doi.org/10.1117/12.764043
Winkler AM, Rice PFS, Troutman TS, Backer MV, Backer JM, Drezek RA et al. Fluorescent and scattering contrast agents in a mouse model of colorectal cancer. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6851. 2008. 68510L https://doi.org/10.1117/12.764043
Winkler, Amy M. ; Rice, Photini F S ; Troutman, Timothy S. ; Backer, Marina V. ; Backer, Joseph M. ; Drezek, Rebekah A. ; Romanowski, Marek ; Barton, Jennifer K. / Fluorescent and scattering contrast agents in a mouse model of colorectal cancer. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6851 2008.
@inproceedings{ca543faf191c4f7c964ff5087a7c4326,
title = "Fluorescent and scattering contrast agents in a mouse model of colorectal cancer",
abstract = "In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..",
keywords = "Colon cancer, Laser-induced fluorescence, Optical coherence tomography",
author = "Winkler, {Amy M.} and Rice, {Photini F S} and Troutman, {Timothy S.} and Backer, {Marina V.} and Backer, {Joseph M.} and Drezek, {Rebekah A.} and Marek Romanowski and Barton, {Jennifer K}",
year = "2008",
doi = "10.1117/12.764043",
language = "English (US)",
isbn = "9780819470263",
volume = "6851",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Fluorescent and scattering contrast agents in a mouse model of colorectal cancer

AU - Winkler, Amy M.

AU - Rice, Photini F S

AU - Troutman, Timothy S.

AU - Backer, Marina V.

AU - Backer, Joseph M.

AU - Drezek, Rebekah A.

AU - Romanowski, Marek

AU - Barton, Jennifer K

PY - 2008

Y1 - 2008

N2 - In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..

AB - In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..

KW - Colon cancer

KW - Laser-induced fluorescence

KW - Optical coherence tomography

UR - http://www.scopus.com/inward/record.url?scp=42149125612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149125612&partnerID=8YFLogxK

U2 - 10.1117/12.764043

DO - 10.1117/12.764043

M3 - Conference contribution

AN - SCOPUS:42149125612

SN - 9780819470263

VL - 6851

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -