Fluorescent and scattering contrast agents in a mouse model of colorectal cancer

Amy M. Winkler, Photini F.S. Rice, Timothy S. Troutman, Marina V. Backer, Joseph M. Backer, Rebekah A. Drezek, Marek Romanowski, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy III
DOIs
StatePublished - Apr 21 2008
EventEndoscopic Microscopy III - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6851
ISSN (Print)1605-7422

Other

OtherEndoscopic Microscopy III
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

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Keywords

  • Colon cancer
  • Laser-induced fluorescence
  • Optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Winkler, A. M., Rice, P. F. S., Troutman, T. S., Backer, M. V., Backer, J. M., Drezek, R. A., Romanowski, M., & Barton, J. K. (2008). Fluorescent and scattering contrast agents in a mouse model of colorectal cancer. In Endoscopic Microscopy III [68510L] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6851). https://doi.org/10.1117/12.764043