Fluorescent markers for studying phagosome-lysosome fusion

M. B. Goren, C. L. Swendson, J. Fiscus, C. Miranti

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Lysosomotropic fluorescent aminoacridines such as acridine orange and quinacrine have achieved prominence as markers for studying lysosome-phagosome fusion, especially in macrophages. Experiments described demonstrate that because the aminoacridines traverse biological membranes with facility, they diffuse throughout the system, and ultimately accumulate intra- or extra-cellularly where they are most efficiently bound. Their presence or absence in phagosomes is therefore not unequivocally indicative of fusion or nonfusion. Alternative fluorescent lysosomal markers are described, and systems defined for which the aminoacridines may probably be used with confidence.

Original languageEnglish (US)
Pages (from-to)273-292
Number of pages20
JournalJournal of Leukocyte Biology
Volume36
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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