Folate functionalized PLGA nanoparticles loaded with plasmid pVAX1-NH36: Mathematical analysis of release

Cindy Alejandra Gutiérrez-Valenzuela, Patricia Guerrero-Germán, Armando Tejeda-Mansir, Reynaldo Esquivel, Roberto Guzmán-Z, Armando Lucero-Acuña

Research output: Contribution to journalArticlepeer-review

Abstract

Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-coglycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below -24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.

Original languageEnglish (US)
Article number364
JournalApplied Sciences (Switzerland)
Volume6
Issue number12
DOIs
StatePublished - 2016

Keywords

  • Copolymer synthesis
  • Drug delivery
  • Folate nanoparticles
  • Leishmaniasis
  • Mathematical analysis
  • PVAX1-NH36

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

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