Forskolin stimulation of water and cation permeability in aquaporin 1 water channels

Andrea J. Yool, W. Daniel Stamer, John W Regan

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Aquaporin1, a six-transmembrane domain protein, is a water channel present in many fluid-secreting and -absorbing cells. In Xenopus oocytes injected with aquaporin 1 complementary RNA, the application of forskolin or cyclic 8-bromo-adenosine 3',5'-monophosphate increased membrane permeability to water and triggered a cationic conductance. The cationic conductance was also induced by direct injection of protein kinase A (PKA) catalytic subunit, reduced by the kinase inhibitor H7, and blocked by HgCl2, an inhibitor of aquaporin 1. The cationic permeability of the aquaporin 1 channel is activated by a cyclic adenosine monophosphate-dependent mechanism that may involve direct or indirect phosphorylation by PKA.

Original languageEnglish (US)
Pages (from-to)1216-1218
Number of pages3
JournalScience
Volume273
Issue number5279
StatePublished - 1996

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Aquaporin 1
Aquaporins
Colforsin
Cations
Permeability
Cyclic AMP-Dependent Protein Kinases
Water
Complementary RNA
Mercuric Chloride
Xenopus
Cyclic AMP
Adenosine
Oocytes
Catalytic Domain
Phosphotransferases
Phosphorylation
Injections
Membranes

ASJC Scopus subject areas

  • General

Cite this

Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. / Yool, Andrea J.; Stamer, W. Daniel; Regan, John W.

In: Science, Vol. 273, No. 5279, 1996, p. 1216-1218.

Research output: Contribution to journalArticle

Yool, Andrea J. ; Stamer, W. Daniel ; Regan, John W. / Forskolin stimulation of water and cation permeability in aquaporin 1 water channels. In: Science. 1996 ; Vol. 273, No. 5279. pp. 1216-1218.
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