Four-channel optical detection on protein-patterned BioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a four-channel detection method on a protein-patterned BioCD that simultaneously measures fluorescence, Rayleigh scattering and/or diffraction, and two interferometric channels in orthogonal quadratures: one that measures differential phase and the other that measures direct phase. The latter two channels constitute label-free interferometric protein detection, while fluorescence and Mie scattering detection provide complementary tools. The BioCD is constructed as dielectric coated disks. Protein molecules patterned on dielectric films change the Fresnel reflection coefficient of the films. The change is exhibited in two ways: the reflection coefficient and the phase are both modulated. These are detected simultaneously by a split detector and designated as "amplitude" signal and "phase contrast" (PC) signal. We are able to scan and image patterned proteins across an entire coated disk with high speed in four channels. A single-analyte immunoassay shows strong correlation between the fluorescence channel and the interferometry channel with a detection limit of 10 ng/ml in a complex protein background (rat lysate) concentration of 7 mg/ml.

Original languageEnglish (US)
Title of host publicationNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
DOIs
StatePublished - Apr 30 2007
EventNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV - San Jose, CA, United States
Duration: Jan 23 2007Jan 24 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6447
ISSN (Print)1605-7422

Other

OtherNanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period1/23/071/24/07

Keywords

  • BioCD
  • Bleaching
  • Fluorescence
  • Immunoassay
  • Microarray
  • Mie scattering
  • Optical biosensor
  • Quenching
  • Spinning-disk interferometry

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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