Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings

Benjamin A. Heitz, Ian W. Jones, Henry K. Hall, Craig A Aspinwall, Steven S Saavedra

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Suspended planar lipid membranes (or black lipid membranes (BLMs)) are widely used for studying reconstituted ion channels, although they lack the chemical and mechanical stability needed for incorporation into high-throughput biosensors and biochips. Lipid polymerization enhances BLM stability but is incompatible with ion channel function when membrane fluidity is required. Here, we demonstrate the preparation of a highly stable BLM that retains significant fluidity by using a mixture of polymerizable and nonpolymerizable phospholipids. Alamethicin, a voltage-gated peptide channel for which membrane fluidity is required for activity, was reconstituted into mixed BLMs prepared using bis-dienoyl phosphatidylcholine (bis-DenPC) and diphytanoyl phosphatidylcholine (DPhPC). Polymerization yielded BLMs that retain the fluidity required for alamethicin activity yet are stable for several days as compared to a few hours prior to polymerization. Thus, these polymerized, binary composition BLMs feature both fluidity and long-term stability.

Original languageEnglish (US)
Pages (from-to)7086-7093
Number of pages8
JournalJournal of the American Chemical Society
Volume132
Issue number20
DOIs
StatePublished - May 26 2010

Fingerprint

Membrane Lipids
Ion Channels
Polymerization
Fluidity
Membranes
Membrane Fluidity
Fluids
Ions
Alamethicin
Phosphatidylcholines
Lipids
Biochips
Biosensing Techniques
Mechanical stability
Phospholipids
Chemical stability
Biosensors
Peptides
Throughput
Electric potential

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings. / Heitz, Benjamin A.; Jones, Ian W.; Hall, Henry K.; Aspinwall, Craig A; Saavedra, Steven S.

In: Journal of the American Chemical Society, Vol. 132, No. 20, 26.05.2010, p. 7086-7093.

Research output: Contribution to journalArticle

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