Fragile X protein controls the efficacy of mRNA transport in Drosophila neurons

Patricia S. Estes, Michele O'Shea, Sara Clasen, Daniela C Zarnescu

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Fragile X syndrome, the most common form of inherited mental retardation is caused by mutations in the FMR1 gene. FMR1 encodes an RNA-binding protein thought to control the transport and translation of target mRNAs. While the function of FMRP in translational control has been clearly demonstrated, its role in mRNA transport and localization in neurons remains elusive. Using a genetically encoded mRNA imaging system in Drosophila we provide the first demonstration that FMRP controls mRNA transport. Live imaging of FMRP associated mRNAs show that mRNA granules are less motile and exhibit decreased directional movement in dFmr1 mutant neurons. Furthermore, Fluorescence Recovery After Photobleaching experiments show that the mobile fraction of mRNA molecules within neurites is dependent on FMRP dosage. These data support a model whereby FMRP regulates transport efficacy, by regulating the association between mRNA cargo and microtubules and suggest a new mechanism for the disease. Crown

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume39
Issue number2
DOIs
StatePublished - Oct 2008

Fingerprint

Drosophila
Neurons
Messenger RNA
Proteins
Fluorescence Recovery After Photobleaching
Fragile X Syndrome
RNA-Binding Proteins
Protein Biosynthesis
Neurites
Crowns
Microtubules
Intellectual Disability
Mutation
Genes

Keywords

  • Drosophila
  • Fragile X protein
  • Live imaging
  • mRNA transport
  • Neurons

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Fragile X protein controls the efficacy of mRNA transport in Drosophila neurons. / Estes, Patricia S.; O'Shea, Michele; Clasen, Sara; Zarnescu, Daniela C.

In: Molecular and Cellular Neuroscience, Vol. 39, No. 2, 10.2008, p. 170-179.

Research output: Contribution to journalArticle

Estes, Patricia S. ; O'Shea, Michele ; Clasen, Sara ; Zarnescu, Daniela C. / Fragile X protein controls the efficacy of mRNA transport in Drosophila neurons. In: Molecular and Cellular Neuroscience. 2008 ; Vol. 39, No. 2. pp. 170-179.
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