Freeze fracture of monolayer cultures

B. U. Pauli, Ronald S Weinstein, L. W. Soble, J. Alroy

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

This paper describes a simple method for the freeze fracturing of cells in monolayers or multilayer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze fracturing.

Original languageEnglish (US)
Pages (from-to)763-769
Number of pages7
JournalJournal of Cell Biology
Volume72
Issue number3
StatePublished - 1977
Externally publishedYes

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Freeze Fracturing
Organ Culture Techniques
Equipment and Supplies

ASJC Scopus subject areas

  • Cell Biology

Cite this

Pauli, B. U., Weinstein, R. S., Soble, L. W., & Alroy, J. (1977). Freeze fracture of monolayer cultures. Journal of Cell Biology, 72(3), 763-769.

Freeze fracture of monolayer cultures. / Pauli, B. U.; Weinstein, Ronald S; Soble, L. W.; Alroy, J.

In: Journal of Cell Biology, Vol. 72, No. 3, 1977, p. 763-769.

Research output: Contribution to journalArticle

Pauli, BU, Weinstein, RS, Soble, LW & Alroy, J 1977, 'Freeze fracture of monolayer cultures', Journal of Cell Biology, vol. 72, no. 3, pp. 763-769.
Pauli BU, Weinstein RS, Soble LW, Alroy J. Freeze fracture of monolayer cultures. Journal of Cell Biology. 1977;72(3):763-769.
Pauli, B. U. ; Weinstein, Ronald S ; Soble, L. W. ; Alroy, J. / Freeze fracture of monolayer cultures. In: Journal of Cell Biology. 1977 ; Vol. 72, No. 3. pp. 763-769.
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