Freeze fracture of monolayer cultures

B. U. Pauli, Ronald S Weinstein, L. W. Soble, J. Alroy

Research output: Contribution to journalArticle

74 Scopus citations


This paper describes a simple method for the freeze fracturing of cells in monolayers or multilayer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze fracturing.

Original languageEnglish (US)
Pages (from-to)763-769
Number of pages7
JournalJournal of Cell Biology
Issue number3
Publication statusPublished - 1977
Externally publishedYes


ASJC Scopus subject areas

  • Cell Biology

Cite this

Pauli, B. U., Weinstein, R. S., Soble, L. W., & Alroy, J. (1977). Freeze fracture of monolayer cultures. Journal of Cell Biology, 72(3), 763-769.