Frequent occurrence of deletions in primary mediastinal B-cell lymphoma

Lindsey R. Kimm, Ronald J. DeLeeuw, Kerry J. Savage, Andreas Rosenwald, Elias Campo, Jan Delabie, German Ott, Hans Konrad Muller-Hermelink, Elaine S. Jaffe, Lisa M Rimsza, Dennis D. Weisenburger, Wing C. Chan, Louis M. Staudt, Joseph M. Connors, Randy D. Gascoyne, Wan L. Lam

Research output: Contribution to journalArticle

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Abstract

Primary mediastinal B-cell lymphoma (PMBCL) is a distinct subtype of diffuse large B-cell lymphoma. PMBCL has been previously studied with a variety of genomic techniques resulting in frequent detection of chromosomal gains; however, chromosomal losses have been rarely reported. This finding contrasts many other types of lymphoma, in which deletions are common. We hypothesize that segmental losses do exist but may have escaped detection by methods used in the previous studies. Using array comparative genomic hybridization to a tiling-resolution microarray encompassing the entire human genome, PMBCL samples were analyzed for genomic copy number alterations. An almost equal number of gains and losses of chromosomal material were detected throughout the genome (216 vs. 193, respectively). A selection of these DNA copy number alterations were confirmed by quantitative real-time PCR. Recurrent gains were detected at all previously reported regions of gain, including 9p seen in ∼70% of cases. Recurrent chromosomal losses were observed at 1p, 3p, 4q, 6q, 7p, and 17p, with a novel event at 1p13.1-p13.2 representing the most frequent at 42% of cases analyzed. We conclude that consistent losses are present in the PMBCL genome. Given the similar frequency of losses to that of segmental gains of DNA, they are likely to play an important role in the pathogenesis of PMBCL.

Original languageEnglish (US)
Pages (from-to)1090-1097
Number of pages8
JournalGenes Chromosomes and Cancer
Volume46
Issue number12
DOIs
StatePublished - Dec 2007

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B-Cell Lymphoma
Genome
Comparative Genomic Hybridization
Lymphoma, Large B-Cell, Diffuse
DNA
Human Genome
Real-Time Polymerase Chain Reaction
Lymphoma

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Kimm, L. R., DeLeeuw, R. J., Savage, K. J., Rosenwald, A., Campo, E., Delabie, J., ... Lam, W. L. (2007). Frequent occurrence of deletions in primary mediastinal B-cell lymphoma. Genes Chromosomes and Cancer, 46(12), 1090-1097. https://doi.org/10.1002/gcc.20495

Frequent occurrence of deletions in primary mediastinal B-cell lymphoma. / Kimm, Lindsey R.; DeLeeuw, Ronald J.; Savage, Kerry J.; Rosenwald, Andreas; Campo, Elias; Delabie, Jan; Ott, German; Muller-Hermelink, Hans Konrad; Jaffe, Elaine S.; Rimsza, Lisa M; Weisenburger, Dennis D.; Chan, Wing C.; Staudt, Louis M.; Connors, Joseph M.; Gascoyne, Randy D.; Lam, Wan L.

In: Genes Chromosomes and Cancer, Vol. 46, No. 12, 12.2007, p. 1090-1097.

Research output: Contribution to journalArticle

Kimm, LR, DeLeeuw, RJ, Savage, KJ, Rosenwald, A, Campo, E, Delabie, J, Ott, G, Muller-Hermelink, HK, Jaffe, ES, Rimsza, LM, Weisenburger, DD, Chan, WC, Staudt, LM, Connors, JM, Gascoyne, RD & Lam, WL 2007, 'Frequent occurrence of deletions in primary mediastinal B-cell lymphoma', Genes Chromosomes and Cancer, vol. 46, no. 12, pp. 1090-1097. https://doi.org/10.1002/gcc.20495
Kimm LR, DeLeeuw RJ, Savage KJ, Rosenwald A, Campo E, Delabie J et al. Frequent occurrence of deletions in primary mediastinal B-cell lymphoma. Genes Chromosomes and Cancer. 2007 Dec;46(12):1090-1097. https://doi.org/10.1002/gcc.20495
Kimm, Lindsey R. ; DeLeeuw, Ronald J. ; Savage, Kerry J. ; Rosenwald, Andreas ; Campo, Elias ; Delabie, Jan ; Ott, German ; Muller-Hermelink, Hans Konrad ; Jaffe, Elaine S. ; Rimsza, Lisa M ; Weisenburger, Dennis D. ; Chan, Wing C. ; Staudt, Louis M. ; Connors, Joseph M. ; Gascoyne, Randy D. ; Lam, Wan L. / Frequent occurrence of deletions in primary mediastinal B-cell lymphoma. In: Genes Chromosomes and Cancer. 2007 ; Vol. 46, No. 12. pp. 1090-1097.
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AU - Weisenburger, Dennis D.

AU - Chan, Wing C.

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AU - Lam, Wan L.

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