Freshly isolated bone marrow cells induce death of various carcinoma cell lines

Nicolas Larmonier, François Ghiringhellii, Claire B Larmonier, Monique Moutet, Annie Fromentin, Emmanuel Baulot, Eric Solary, Bernard Bonnotte, François Martin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In some carcinomas such as digestive tract carcinomas, bone marrow infiltration by tumor cells is a frequent event but usually remains a micrometastatic disease and rarely induces overt bone lesions. The mechanisms responsible for the control of these metastases in the bone marrow remain poorly known. We show that freshly isolated bone marrow cells from human, murine and rat origin rapidly kill a wide range of syngeneic or xenogeneic carcinoma cell lines in culture. Further analysis of this cytotoxic process in the rat indicated that neither resident bone marrow macrophages nor NK cells were responsible for this cytotoxic effect that was restricted to a subpopulation of bone marrow cells expressing CD90 (Thy-1), a marker of hemopoietic precursors. The tumoricidal activity of these cells did not require long-term culture nor addition of exogenous cytokines or growth factors. A subset of CD90+ cells that rapidly differentiates into CD163(ED2)-expressing macrophages was observed to be responsible for tumor cell killing. These macrophages induced a non-apoptotic death of tumor cells, a process that required both a direct interaction with the tumor cell and nitric oxide (NO) production through the activation of inducible nitric oxide-synthase (iNOS). This ability of pluripotent hemopoietic stem cells to rapidly differentiate into macrophages capable of killing invasive tumor cells may account for the limited expansion of micrometastases of some carcinomas in the bone marrow.

Original languageEnglish (US)
Pages (from-to)747-756
Number of pages10
JournalInternational Journal of Cancer
Volume107
Issue number5
DOIs
StatePublished - Dec 10 2003
Externally publishedYes

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Bone Marrow Cells
Cell Death
Carcinoma
Cell Line
Bone Marrow
Macrophages
Neoplasms
Neoplasm Micrometastasis
Pluripotent Stem Cells
Nitric Oxide Synthase Type II
Natural Killer Cells
Gastrointestinal Tract
Intercellular Signaling Peptides and Proteins
Nitric Oxide
Cytokines
Neoplasm Metastasis
Bone and Bones

Keywords

  • Bone marrow
  • Carcinoma
  • Cytotoxicity
  • Differentiation
  • Macrophages

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Freshly isolated bone marrow cells induce death of various carcinoma cell lines. / Larmonier, Nicolas; Ghiringhellii, François; Larmonier, Claire B; Moutet, Monique; Fromentin, Annie; Baulot, Emmanuel; Solary, Eric; Bonnotte, Bernard; Martin, François.

In: International Journal of Cancer, Vol. 107, No. 5, 10.12.2003, p. 747-756.

Research output: Contribution to journalArticle

Larmonier, N, Ghiringhellii, F, Larmonier, CB, Moutet, M, Fromentin, A, Baulot, E, Solary, E, Bonnotte, B & Martin, F 2003, 'Freshly isolated bone marrow cells induce death of various carcinoma cell lines', International Journal of Cancer, vol. 107, no. 5, pp. 747-756. https://doi.org/10.1002/ijc.11463
Larmonier, Nicolas ; Ghiringhellii, François ; Larmonier, Claire B ; Moutet, Monique ; Fromentin, Annie ; Baulot, Emmanuel ; Solary, Eric ; Bonnotte, Bernard ; Martin, François. / Freshly isolated bone marrow cells induce death of various carcinoma cell lines. In: International Journal of Cancer. 2003 ; Vol. 107, No. 5. pp. 747-756.
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