FTIR spectroscopic study of biogenic Mn-oxide formation by Pseudomonas putida GB-1

Sanjai J. Parikh, Jon Chorover

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnOx, whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., "acid" birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnOx accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.

Original languageEnglish (US)
Pages (from-to)207-218
Number of pages12
JournalGeomicrobiology Journal
Volume22
Issue number5
DOIs
StatePublished - Jul 2005

Fingerprint

Pseudomonas putida
Fourier Analysis
Biofilms
Cell Adhesion
Oxides
Fourier transform
Fourier transforms
adhesion
oxide
Infrared radiation
Cell adhesion
oxidation
Oxidation
biofilm
reflectance
Phosphates
Carbohydrates
Proteins
protein
Fourier Transform Infrared Spectroscopy

Keywords

  • Bacterial adhesion
  • Biomineralization
  • FTIR spectroscopy
  • Mn oxidizing bacteria
  • Mn(IV) oxides
  • Pseudomonas putida

ASJC Scopus subject areas

  • Earth and Planetary Sciences (miscellaneous)
  • Microbiology
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

FTIR spectroscopic study of biogenic Mn-oxide formation by Pseudomonas putida GB-1. / Parikh, Sanjai J.; Chorover, Jon.

In: Geomicrobiology Journal, Vol. 22, No. 5, 07.2005, p. 207-218.

Research output: Contribution to journalArticle

@article{9de2fe820a604582b851911eebd4c877,
title = "FTIR spectroscopic study of biogenic Mn-oxide formation by Pseudomonas putida GB-1",
abstract = "Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnOx, whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., {"}acid{"} birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnOx accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.",
keywords = "Bacterial adhesion, Biomineralization, FTIR spectroscopy, Mn oxidizing bacteria, Mn(IV) oxides, Pseudomonas putida",
author = "Parikh, {Sanjai J.} and Jon Chorover",
year = "2005",
month = "7",
doi = "10.1080/01490450590947724",
language = "English (US)",
volume = "22",
pages = "207--218",
journal = "Geomicrobiology Journal",
issn = "0149-0451",
publisher = "Taylor and Francis Ltd.",
number = "5",

}

TY - JOUR

T1 - FTIR spectroscopic study of biogenic Mn-oxide formation by Pseudomonas putida GB-1

AU - Parikh, Sanjai J.

AU - Chorover, Jon

PY - 2005/7

Y1 - 2005/7

N2 - Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnOx, whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., "acid" birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnOx accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.

AB - Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnOx, whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., "acid" birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnOx accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.

KW - Bacterial adhesion

KW - Biomineralization

KW - FTIR spectroscopy

KW - Mn oxidizing bacteria

KW - Mn(IV) oxides

KW - Pseudomonas putida

UR - http://www.scopus.com/inward/record.url?scp=21344452069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21344452069&partnerID=8YFLogxK

U2 - 10.1080/01490450590947724

DO - 10.1080/01490450590947724

M3 - Article

AN - SCOPUS:21344452069

VL - 22

SP - 207

EP - 218

JO - Geomicrobiology Journal

JF - Geomicrobiology Journal

SN - 0149-0451

IS - 5

ER -