Fucoidin reduces coronary microvascular leukocyte accumulation early in reperfusion

Leslie S Ritter, Jack G. Copeland, Paul F. McDonagh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background. Leukocytes rapidly accumulate in the heart early in reperfusion after ischemia, contributing to reperfusion injury. The purpose of this study was to determine whether treatment with the selectin blocker fucoidin (FCN) would attenuate early leukocyte retention in coronary venules and capillaries during low flow reperfusion. Methods. Isolated rat hearts subjected to 30 minutes of 37°C, no-flow ischemia were initially reperfused with blood containing labeled leukocytes, followed by reperfusion with a Krebs red cell solution. The deposition of leukocytes in coronary capillaries and venules was observed using intravital microscopy. Three groups were studied: nonischemic control hearts, untreated postischemic hearts reperfused at low flow, and postischemic hearts reperfused at low flow, where both the hearts and the blood reperfusate were pretreated with FCN (0.36 mg/mL blood). Results. In the ischemia-reperfusion group, we observed a rapid and significant increase in leukocyte accumulation in both capillaries and venules. Treatment with FCN significantly reduced the leukocyte accumulation in both capillaries and venules (p < 0.05). In addition, FCN significantly reduced the persistence of leukostasis in both capillaries and venules, indicating that FCN affected a transient adhesion process. Conclusions. These results suggest that the selectin family of leukocyte-endothelial cell adhesion proteins mediates the initial retention of leukocytes in both coronary capillaries and venules during reperfusion. Selectin blockade may be effective in reducing the contribution of leukocytes to early reperfusion injury.

Original languageEnglish (US)
Pages (from-to)2063-2072
Number of pages10
JournalAnnals of Thoracic Surgery
Volume66
Issue number6
DOIs
StatePublished - 1998

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Reperfusion
Leukocytes
Venules
Selectins
Ischemia
Reperfusion Injury
Leukostasis
fucoidan
Cell Adhesion
Endothelial Cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Fucoidin reduces coronary microvascular leukocyte accumulation early in reperfusion. / Ritter, Leslie S; Copeland, Jack G.; McDonagh, Paul F.

In: Annals of Thoracic Surgery, Vol. 66, No. 6, 1998, p. 2063-2072.

Research output: Contribution to journalArticle

Ritter, Leslie S ; Copeland, Jack G. ; McDonagh, Paul F. / Fucoidin reduces coronary microvascular leukocyte accumulation early in reperfusion. In: Annals of Thoracic Surgery. 1998 ; Vol. 66, No. 6. pp. 2063-2072.
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