Functional characterization of the H-2Dp gene product

Elizabeth McLaughlin-Taylor, Jerold G. Woodward, Mary J. Macchi, Minnie McMillan, Jeffrey A Frelinger

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A λ clone containing the Dp gene was used to transform L cells. The Dp product expressed was identified by two-dimensional gel electrophoreis and flow cytometry. The Dp product expressed by the L cells was recognized by DP-specific flow cytometry. The Dp product expressed by the L cells was recognized by Dp-specific but not Kp-specific killer T cells. This killing was inhibited by monoclonal antibodies specific for Dp but not Kp or Kk antigens. Similarly, lymphocytic choriomeningitis virus (LCMV) killer T cells from B 10.P mice were able to kill LCMV-infected L12a cells, but not LCMV-infected Ltk+. Again only Dp monoclonal antibodies could inhibit this killing.

Original languageEnglish (US)
Pages (from-to)205-216
Number of pages12
JournalImmunogenetics
Volume19
Issue number3
DOIs
StatePublished - Mar 1984
Externally publishedYes

Fingerprint

Lymphocytic choriomeningitis virus
Genes
Flow Cytometry
Monoclonal Antibodies
T-Lymphocytes
Clone Cells
Gels
Antigens

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

McLaughlin-Taylor, E., Woodward, J. G., Macchi, M. J., McMillan, M., & Frelinger, J. A. (1984). Functional characterization of the H-2Dp gene product. Immunogenetics, 19(3), 205-216. https://doi.org/10.1007/BF00364764

Functional characterization of the H-2Dp gene product. / McLaughlin-Taylor, Elizabeth; Woodward, Jerold G.; Macchi, Mary J.; McMillan, Minnie; Frelinger, Jeffrey A.

In: Immunogenetics, Vol. 19, No. 3, 03.1984, p. 205-216.

Research output: Contribution to journalArticle

McLaughlin-Taylor, E, Woodward, JG, Macchi, MJ, McMillan, M & Frelinger, JA 1984, 'Functional characterization of the H-2Dp gene product', Immunogenetics, vol. 19, no. 3, pp. 205-216. https://doi.org/10.1007/BF00364764
McLaughlin-Taylor E, Woodward JG, Macchi MJ, McMillan M, Frelinger JA. Functional characterization of the H-2Dp gene product. Immunogenetics. 1984 Mar;19(3):205-216. https://doi.org/10.1007/BF00364764
McLaughlin-Taylor, Elizabeth ; Woodward, Jerold G. ; Macchi, Mary J. ; McMillan, Minnie ; Frelinger, Jeffrey A. / Functional characterization of the H-2Dp gene product. In: Immunogenetics. 1984 ; Vol. 19, No. 3. pp. 205-216.
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