Functional characterization of the H-2Dp gene product

Elizabeth McLaughlin-Taylor, Jerold G. Woodward, Mary J. Macchi, Minnie McMillan, Jeffrey A. Frelinger

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A λ clone containing the Dp gene was used to transform L cells. The Dp product expressed was identified by two-dimensional gel electrophoreis and flow cytometry. The Dp product expressed by the L cells was recognized by DP-specific flow cytometry. The Dp product expressed by the L cells was recognized by Dp-specific but not Kp-specific killer T cells. This killing was inhibited by monoclonal antibodies specific for Dp but not Kp or Kk antigens. Similarly, lymphocytic choriomeningitis virus (LCMV) killer T cells from B 10.P mice were able to kill LCMV-infected L12a cells, but not LCMV-infected Ltk+. Again only Dp monoclonal antibodies could inhibit this killing.

Original languageEnglish (US)
Pages (from-to)205-216
Number of pages12
JournalImmunogenetics
Volume19
Issue number3
DOIs
StatePublished - Mar 1984

ASJC Scopus subject areas

  • Immunology
  • Genetics

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