Functional Interaction of p53 and BLM DNA Helicase in Apoptosis

Xin Wei Wang, Ann Tseng, Nathan Ellis, Elisa A. Spillare, Steven P. Linke, Ana I. Robles, Hasan Seker, Qin Yang, Peng Hu, Sergey Beresten, Nicole A. Bemmels, Susan Garfield, Curtis C. Harris

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

The Bloom syndrome (BS) protein, BLM, is a member of the RecQ DNA helicase family that also includes the Werner syndrome protein, WRN. Inherited mutations in these proteins are associated with cancer predisposition of these patients. We recently discovered that cells from Werner syndrome patients displayed a deficiency in p53-mediated apoptosis and WRN binds to p53. Here, we report that analogous to WRN, BLM also binds to p53 in vivo and in vitro, and the C-terminal domain of p53 is responsible for the interaction. p53-mediated apoptosis is defective in BS fibroblasts and can be rescued by expression of the normal BLM gene. Moreover, lymphoblastoid cell lines (LCLs) derived from BS donors are resistant to both γ-radiation and doxorubicin-induced cell killing, and sensitivity can be restored by the stable expression of normal BLM. In contrast, BS cells have a normal Fas-mediated apoptosis, and in response to DNA damage normal accumulation of p53, normal induction of p53 responsive genes, and normal G1-S and G2-M cell cycle arrest. BLM localizes to nuclear foci referred to as PML nuclear bodies (NBs). Cells from Li-Fraumeni syndrome patients carrying p53 germline mutations and LCLs lacking a functional p53 have a decreased accumulation of BLM in NBs, whereas isogenic lines with functional p53 exhibit normal accumulation. Certain BLM mutants (C1055S or Δ133-237) that have a reduced ability to localize to the NBs when expressed in normal cells can impair the localization of wild type BLM to NBs and block p53-mediated apoptosis, suggesting a dominant-negative effect. Taken together, our results indicate both a novel mechanism of p53 function by which p53 mediates nuclear trafficking of BLM to NBs and the cooperation of p53 and BLM to induce apoptosis.

Original languageEnglish (US)
Pages (from-to)32948-32955
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number35
DOIs
StatePublished - Aug 31 2001
Externally publishedYes

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DNA Helicases
Bloom Syndrome
Apoptosis
Werner Syndrome
Cells
Genes
RecQ Helicases
Li-Fraumeni Syndrome
G2 Phase Cell Cycle Checkpoints
Cell Line
Germ-Line Mutation
p53 Genes
Fibroblasts
Doxorubicin
DNA Damage
Proteins
Tissue Donors
Radiation
Mutation
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wang, X. W., Tseng, A., Ellis, N., Spillare, E. A., Linke, S. P., Robles, A. I., ... Harris, C. C. (2001). Functional Interaction of p53 and BLM DNA Helicase in Apoptosis. Journal of Biological Chemistry, 276(35), 32948-32955. https://doi.org/10.1074/jbc.M103298200

Functional Interaction of p53 and BLM DNA Helicase in Apoptosis. / Wang, Xin Wei; Tseng, Ann; Ellis, Nathan; Spillare, Elisa A.; Linke, Steven P.; Robles, Ana I.; Seker, Hasan; Yang, Qin; Hu, Peng; Beresten, Sergey; Bemmels, Nicole A.; Garfield, Susan; Harris, Curtis C.

In: Journal of Biological Chemistry, Vol. 276, No. 35, 31.08.2001, p. 32948-32955.

Research output: Contribution to journalArticle

Wang, XW, Tseng, A, Ellis, N, Spillare, EA, Linke, SP, Robles, AI, Seker, H, Yang, Q, Hu, P, Beresten, S, Bemmels, NA, Garfield, S & Harris, CC 2001, 'Functional Interaction of p53 and BLM DNA Helicase in Apoptosis', Journal of Biological Chemistry, vol. 276, no. 35, pp. 32948-32955. https://doi.org/10.1074/jbc.M103298200
Wang XW, Tseng A, Ellis N, Spillare EA, Linke SP, Robles AI et al. Functional Interaction of p53 and BLM DNA Helicase in Apoptosis. Journal of Biological Chemistry. 2001 Aug 31;276(35):32948-32955. https://doi.org/10.1074/jbc.M103298200
Wang, Xin Wei ; Tseng, Ann ; Ellis, Nathan ; Spillare, Elisa A. ; Linke, Steven P. ; Robles, Ana I. ; Seker, Hasan ; Yang, Qin ; Hu, Peng ; Beresten, Sergey ; Bemmels, Nicole A. ; Garfield, Susan ; Harris, Curtis C. / Functional Interaction of p53 and BLM DNA Helicase in Apoptosis. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 35. pp. 32948-32955.
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abstract = "The Bloom syndrome (BS) protein, BLM, is a member of the RecQ DNA helicase family that also includes the Werner syndrome protein, WRN. Inherited mutations in these proteins are associated with cancer predisposition of these patients. We recently discovered that cells from Werner syndrome patients displayed a deficiency in p53-mediated apoptosis and WRN binds to p53. Here, we report that analogous to WRN, BLM also binds to p53 in vivo and in vitro, and the C-terminal domain of p53 is responsible for the interaction. p53-mediated apoptosis is defective in BS fibroblasts and can be rescued by expression of the normal BLM gene. Moreover, lymphoblastoid cell lines (LCLs) derived from BS donors are resistant to both γ-radiation and doxorubicin-induced cell killing, and sensitivity can be restored by the stable expression of normal BLM. In contrast, BS cells have a normal Fas-mediated apoptosis, and in response to DNA damage normal accumulation of p53, normal induction of p53 responsive genes, and normal G1-S and G2-M cell cycle arrest. BLM localizes to nuclear foci referred to as PML nuclear bodies (NBs). Cells from Li-Fraumeni syndrome patients carrying p53 germline mutations and LCLs lacking a functional p53 have a decreased accumulation of BLM in NBs, whereas isogenic lines with functional p53 exhibit normal accumulation. Certain BLM mutants (C1055S or Δ133-237) that have a reduced ability to localize to the NBs when expressed in normal cells can impair the localization of wild type BLM to NBs and block p53-mediated apoptosis, suggesting a dominant-negative effect. Taken together, our results indicate both a novel mechanism of p53 function by which p53 mediates nuclear trafficking of BLM to NBs and the cooperation of p53 and BLM to induce apoptosis.",
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AU - Garfield, Susan

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