Functional study of cloned ion channels in mammalian transfection systems using site-directed mutagenesis

Elena E. Brevnova, Bethany H. Parker, Oleksandr Platoshyn, Jason Yuan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The function of ion channels is essential for many cell processes. Functional studies of a particular type of ion channel in native cells are sometimes rather complicated because of the diversity of native channel types. Certain mammalian cell lines developed with very low expression of endogenous ion channels have been used as convenient heterologous expression systems for the functional study of particular ion channels. These systems provide machinery and pathways similar to those of native mammalian cells with normal ion channel expression levels. Furthermore, the heterologous expression approach together with in vitro (or site-directed) mutagenesis allows investigation into protein structure and function targeted to the specific area of interest in an ion channel. The aim of this chapter is to describe the technique of sitedirected mutagenesis and to illustrate how it is applied in functional studies of cloned ion channels heterologously expressed in mammalian cell lines.

Original languageEnglish (US)
Title of host publicationIon Channels in the Pulmonary Vasculature
PublisherCRC Press
Pages687-710
Number of pages24
ISBN (Electronic)9780849350375
ISBN (Print)0824759680, 9780824759681
StatePublished - Jan 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Brevnova, E. E., Parker, B. H., Platoshyn, O., & Yuan, J. (2005). Functional study of cloned ion channels in mammalian transfection systems using site-directed mutagenesis. In Ion Channels in the Pulmonary Vasculature (pp. 687-710). CRC Press.