Fungal communities on speleothem surfaces in Kartchner caverns, Arizona, USA

Michael J. Vaughan, Raina Margaret Maier, Barry M Pryor

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Kartchner Caverns, located near Benson, Arizona, USA, is an active carbonate cave that serves as the major attraction for Kartchner Caverns State Park. Low-impact development and maintenance have preserved prediscovery macroscopic cavern features and minimized disturbances to biological communities within the cave. The goal of this study was to examine fungal diversity in Kartchner Caverns on actively-forming speleothem surfaces. Fifteen formations were sampled from fve sites across the cave. Richness was assessed using standard culture-based fungal isolation techniques. A culture-independent analysis using denaturing gradient gel electrophoresis (DGGE) was used to assay evidence of community homogeneity across the cave through the separation of 18S rDNA amplicons from speleothem community DNA. The culturing effort recovered 53 distinct morphological taxonomic units (MTUs), corresponding to 43 genetic taxonomic units (GTUs) that represented 21 genera. From the observed MTU accumulation curve and the projected total MTU richness curve, it is estimated that 51 percent of the actual MTU richness was recovered. The most commonly isolated fungi belonged to the genera Penicillium, Paecilomyces, Phialophora, and Aspergillus. This culture-based analysis did not reveal signifcant differences in fungal richness or number of fungi recovered across sites. Cluster analysis using DGGE band profles did not reveal distinctive groupings of speleothems by sample site. However, canonical correspondence analysis (CCA) of culture-independent DGGE profles showed a signifcant effect of sampling site and formation type on fungal community structure. Taken together, these results reveal that diverse fungal communities exist on speleothem surfaces in Kartchner Caverns, and that these communities are not uniformly distributed spatially. Analysis of sample saturation indicated that more sampling depth is required to uncover the full scale of mycological richness across spelothem surfaces.

Original languageEnglish (US)
Pages (from-to)65-77
Number of pages13
JournalInternational Journal of Speleology
Volume40
Issue number1
DOIs
StatePublished - 2011

Fingerprint

speleothem
cavern
cave
electrokinesis
gel
fungus
sampling
correspondence analysis
homogeneity
cluster analysis
community structure
assay
saturation
DNA
disturbance
carbonate
analysis

Keywords

  • Arizona
  • DGGE
  • Fungal community
  • Kartchner caverns
  • USA

ASJC Scopus subject areas

  • Geology
  • Earth-Surface Processes

Cite this

Fungal communities on speleothem surfaces in Kartchner caverns, Arizona, USA. / Vaughan, Michael J.; Maier, Raina Margaret; Pryor, Barry M.

In: International Journal of Speleology, Vol. 40, No. 1, 2011, p. 65-77.

Research output: Contribution to journalArticle

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abstract = "Kartchner Caverns, located near Benson, Arizona, USA, is an active carbonate cave that serves as the major attraction for Kartchner Caverns State Park. Low-impact development and maintenance have preserved prediscovery macroscopic cavern features and minimized disturbances to biological communities within the cave. The goal of this study was to examine fungal diversity in Kartchner Caverns on actively-forming speleothem surfaces. Fifteen formations were sampled from fve sites across the cave. Richness was assessed using standard culture-based fungal isolation techniques. A culture-independent analysis using denaturing gradient gel electrophoresis (DGGE) was used to assay evidence of community homogeneity across the cave through the separation of 18S rDNA amplicons from speleothem community DNA. The culturing effort recovered 53 distinct morphological taxonomic units (MTUs), corresponding to 43 genetic taxonomic units (GTUs) that represented 21 genera. From the observed MTU accumulation curve and the projected total MTU richness curve, it is estimated that 51 percent of the actual MTU richness was recovered. The most commonly isolated fungi belonged to the genera Penicillium, Paecilomyces, Phialophora, and Aspergillus. This culture-based analysis did not reveal signifcant differences in fungal richness or number of fungi recovered across sites. Cluster analysis using DGGE band profles did not reveal distinctive groupings of speleothems by sample site. However, canonical correspondence analysis (CCA) of culture-independent DGGE profles showed a signifcant effect of sampling site and formation type on fungal community structure. Taken together, these results reveal that diverse fungal communities exist on speleothem surfaces in Kartchner Caverns, and that these communities are not uniformly distributed spatially. Analysis of sample saturation indicated that more sampling depth is required to uncover the full scale of mycological richness across spelothem surfaces.",
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