TY - JOUR
T1 - Further evidence for the 1,25-dihydroxyvitamin D-like activity of Solanum malacoxylon
AU - Peterlik, M.
AU - Bursac, K.
AU - Haussler, Mark R
AU - Hughes, M. R.
AU - Wasserman, R. H.
PY - 1976/6/7
Y1 - 1976/6/7
N2 - Administration of an aqueous extract of the calcinogenic plant Solanum malacoxylon (S.m.) to vitamin D-deficient or strontium fed chicks produces significant plasma 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) activity within 6 hr. (via radioreceptor assay) and subsequently elicits the appearance of immunoreactive intestinal calcium binding protein. Studies of a purified aqueous extract of S.m. show that it does not compete effectively with radioactive 1,25-(OH)2D3 for binding to the sterol's intestinal receptor. However, treatment of the extract with β-glucosidase releases a biologically active substance which is soluble in organic solvents and efficiently competes with labeled sterol for the receptor. This factor migrates exactly with tritiated 1,25-(OH)2D3 on high resolution Celite liquid-liquid partition columns. Thus, S.m. contains a molecule very similar or identical to 1,25-(OH)2D3 which is combined with one or more carbohydrate moieties in the native plant. This glycoside is probably cleaved in vivo before biological activity is attained.
AB - Administration of an aqueous extract of the calcinogenic plant Solanum malacoxylon (S.m.) to vitamin D-deficient or strontium fed chicks produces significant plasma 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) activity within 6 hr. (via radioreceptor assay) and subsequently elicits the appearance of immunoreactive intestinal calcium binding protein. Studies of a purified aqueous extract of S.m. show that it does not compete effectively with radioactive 1,25-(OH)2D3 for binding to the sterol's intestinal receptor. However, treatment of the extract with β-glucosidase releases a biologically active substance which is soluble in organic solvents and efficiently competes with labeled sterol for the receptor. This factor migrates exactly with tritiated 1,25-(OH)2D3 on high resolution Celite liquid-liquid partition columns. Thus, S.m. contains a molecule very similar or identical to 1,25-(OH)2D3 which is combined with one or more carbohydrate moieties in the native plant. This glycoside is probably cleaved in vivo before biological activity is attained.
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U2 - 10.1016/0006-291X(76)90662-8
DO - 10.1016/0006-291X(76)90662-8
M3 - Article
VL - 70
SP - 797
EP - 804
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -