Further evidence for the 1,25-dihydroxyvitamin D-like activity of Solanum malacoxylon

M. Peterlik, K. Bursac, M. R. Haussler, M. R. Hughes, R. H. Wasserman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Administration of an aqueous extract of the calcinogenic plant Solanum malacoxylon (S.m.) to vitamin D-deficient or strontium fed chicks produces significant plasma 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) activity within 6 hr. (via radioreceptor assay) and subsequently elicits the appearance of immunoreactive intestinal calcium binding protein. Studies of a purified aqueous extract of S.m. show that it does not compete effectively with radioactive 1,25-(OH)2D3 for binding to the sterol's intestinal receptor. However, treatment of the extract with β-glucosidase releases a biologically active substance which is soluble in organic solvents and efficiently competes with labeled sterol for the receptor. This factor migrates exactly with tritiated 1,25-(OH)2D3 on high resolution Celite liquid-liquid partition columns. Thus, S.m. contains a molecule very similar or identical to 1,25-(OH)2D3 which is combined with one or more carbohydrate moieties in the native plant. This glycoside is probably cleaved in vivo before biological activity is attained.

Original languageEnglish (US)
Pages (from-to)797-804
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume70
Issue number3
DOIs
StatePublished - Jun 7 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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