Fused oblique incidence reflectometry and confocal fluorescence microscopy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy VI
DOIs
StatePublished - May 12 2011
EventEndoscopic Microscopy VI - San Francisco, CA, United States
Duration: Jan 23 2011Jan 24 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7893
ISSN (Print)1605-7422

Other

OtherEndoscopic Microscopy VI
CountryUnited States
CitySan Francisco, CA
Period1/23/111/24/11

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Keywords

  • biomedical optics
  • confocal fluorescence microendoscopy
  • oblique incidence
  • optical properties
  • reflectometry
  • scattering spectra
  • tissue optics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Risi, M. D., Rouse, A. R., & Gmitro, A. F. (2011). Fused oblique incidence reflectometry and confocal fluorescence microscopy. In Endoscopic Microscopy VI [78930F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7893). https://doi.org/10.1117/12.875974