Fused oblique incidence reflectometry and confocal fluorescence microscopy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7893
DOIs
StatePublished - 2011
EventEndoscopic Microscopy VI - San Francisco, CA, United States
Duration: Jan 23 2011Jan 24 2011

Other

OtherEndoscopic Microscopy VI
CountryUnited States
CitySan Francisco, CA
Period1/23/111/24/11

Fingerprint

Confocal microscopy
Fluorescence microscopy
Fluorescence Microscopy
Confocal Microscopy
incidence
Optical Imaging
microscopy
fluorescence
fibers
Fibers
Catheters
Incidence
Imaging techniques
bundles
Fluorescence
Tissue
rodents
Fluorophores
Rodentia
Spectrum Analysis

Keywords

  • biomedical optics
  • confocal fluorescence microendoscopy
  • oblique incidence
  • optical properties
  • reflectometry
  • scattering spectra
  • tissue optics

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Risi, M. D., Rouse, A. R., & Gmitro, A. F. (2011). Fused oblique incidence reflectometry and confocal fluorescence microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7893). [78930F] https://doi.org/10.1117/12.875974

Fused oblique incidence reflectometry and confocal fluorescence microscopy. / Risi, Matthew D.; Rouse, Andrew R; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7893 2011. 78930F.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Risi, MD, Rouse, AR & Gmitro, AF 2011, Fused oblique incidence reflectometry and confocal fluorescence microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7893, 78930F, Endoscopic Microscopy VI, San Francisco, CA, United States, 1/23/11. https://doi.org/10.1117/12.875974
Risi MD, Rouse AR, Gmitro AF. Fused oblique incidence reflectometry and confocal fluorescence microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7893. 2011. 78930F https://doi.org/10.1117/12.875974
Risi, Matthew D. ; Rouse, Andrew R ; Gmitro, Arthur F. / Fused oblique incidence reflectometry and confocal fluorescence microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7893 2011.
@inproceedings{03a2b2bcd6d24f22bff87540186b70e9,
title = "Fused oblique incidence reflectometry and confocal fluorescence microscopy",
abstract = "Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.",
keywords = "biomedical optics, confocal fluorescence microendoscopy, oblique incidence, optical properties, reflectometry, scattering spectra, tissue optics",
author = "Risi, {Matthew D.} and Rouse, {Andrew R} and Gmitro, {Arthur F}",
year = "2011",
doi = "10.1117/12.875974",
language = "English (US)",
isbn = "9780819484307",
volume = "7893",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Fused oblique incidence reflectometry and confocal fluorescence microscopy

AU - Risi, Matthew D.

AU - Rouse, Andrew R

AU - Gmitro, Arthur F

PY - 2011

Y1 - 2011

N2 - Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.

AB - Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.

KW - biomedical optics

KW - confocal fluorescence microendoscopy

KW - oblique incidence

KW - optical properties

KW - reflectometry

KW - scattering spectra

KW - tissue optics

UR - http://www.scopus.com/inward/record.url?scp=79955732689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955732689&partnerID=8YFLogxK

U2 - 10.1117/12.875974

DO - 10.1117/12.875974

M3 - Conference contribution

SN - 9780819484307

VL - 7893

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -