G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility

Atsuo T. Sasaki, Chris Janetopoulos, Susan Lee, Pascale G Charest, Kosuke Takeda, Lauren W. Sundheimer, Ruedi Meili, Peter N. Devreotes, Richard A. Firtel

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Phosphoinositide 3-kinase (PI3K)γ and Dictyostelium PI3K are activated via G protein-coupled receptors through binding to the Gβγ subunit and Ras. However, the mechanistic role(s) of Gβγ and Ras in PI3K activation remains elusive. Furthermore, the dynamics and function of PI3K activation in the absence of extracellular stimuli have not been fully investigated. We report that gβ null cells display PI3K and Ras activation, as well as the reciprocal localization of PI3K and PTEN, which lead to local accumulation of PI(3,4,5)P3. Simultaneous imaging analysis reveals that in the absence of extracellular stimuli, autonomous PI3K and Ras activation occur, concurrently, at the same sites where F-actin projection emerges. The loss of PI3K binding to Ras - guanosine triphosphate abolishes this PI3K activation, whereas prevention of PI3K activity suppresses autonomous Ras activation, suggesting that PI3K and Ras form a positive feedback circuit. This circuit is associated with both random cell migration and cytokinesis and may have initially evolved to control stochastic changes in the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)185-191
Number of pages7
JournalJournal of Cell Biology
Volume178
Issue number2
DOIs
StatePublished - Jul 16 2007
Externally publishedYes

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1-Phosphatidylinositol 4-Kinase
GTP-Binding Proteins
Cell Movement
Actins
Null Lymphocytes
Dictyostelium
Cytokinesis
G-Protein-Coupled Receptors
Guanosine Triphosphate
Cytoskeleton

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sasaki, A. T., Janetopoulos, C., Lee, S., Charest, P. G., Takeda, K., Sundheimer, L. W., ... Firtel, R. A. (2007). G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility. Journal of Cell Biology, 178(2), 185-191. https://doi.org/10.1083/jcb.200611138

G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility. / Sasaki, Atsuo T.; Janetopoulos, Chris; Lee, Susan; Charest, Pascale G; Takeda, Kosuke; Sundheimer, Lauren W.; Meili, Ruedi; Devreotes, Peter N.; Firtel, Richard A.

In: Journal of Cell Biology, Vol. 178, No. 2, 16.07.2007, p. 185-191.

Research output: Contribution to journalArticle

Sasaki, AT, Janetopoulos, C, Lee, S, Charest, PG, Takeda, K, Sundheimer, LW, Meili, R, Devreotes, PN & Firtel, RA 2007, 'G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility', Journal of Cell Biology, vol. 178, no. 2, pp. 185-191. https://doi.org/10.1083/jcb.200611138
Sasaki, Atsuo T. ; Janetopoulos, Chris ; Lee, Susan ; Charest, Pascale G ; Takeda, Kosuke ; Sundheimer, Lauren W. ; Meili, Ruedi ; Devreotes, Peter N. ; Firtel, Richard A. / G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility. In: Journal of Cell Biology. 2007 ; Vol. 178, No. 2. pp. 185-191.
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