GABA accumulation causes cell elongation defects and a decrease in expression of genes encoding secreted and cell wall-related proteins in Arabidopsis thaliana

Hugues Renault, Abdelhak El Amrani, Ravishankar Palanivelu, Emily P. Updegraff, Agns Yu, Jean Pierre Renou, Daphne Preuss, Alain Bouchereau, Carole Deleu

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

GABA (γ-aminobutyric acid), a non-protein amino acid, is a signaling factor in many organisms. In plants, GABA is known to accumulate under a variety of stresses. However, the consequence of GABA accumulation, especially in vegetative tissues, remains poorly understood. Moreover, gene expression changes as a consequence of GABA accumulation in plants are largely unknown. The pop2 mutant, which is defective in GABA catabolism and accumulates GABA, is a good model to examine the effects of GABA accumulation on plant development. Here, we show that the pop2 mutants have pollen tube elongation defects in the transmitting tract of pistils. Additionally, we observed growth inhibition of primary root and dark-grown hypocotyl, at least in part due to cell elongation defects, upon exposure to exogenous GABA. Microarray analysis of pop2-1 seedlings grown in GABA-supplemented medium revealed that 60 of genes whose expression decreased encode secreted proteins. Besides, functional classification of genes with decreased expression in the pop2-1 mutant showed that cell wall-related genes were significantly enriched in the microarray data set, consistent with the cell elongation defects observed in pop2 mutants. Our study identifies cell elongation defects caused by GABA accumulation in both reproductive and vegetative tissues. Additionally, our results show that genes that encode secreted and cell wall-related proteins may mediate some of the effects of GABA accumulation. The potential function of GABA as a growth control factor under stressful conditions is discussed.

Original languageEnglish (US)
Pages (from-to)894-908
Number of pages15
JournalPlant and Cell Physiology
Volume52
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Arabidopsis Proteins
gamma-aminobutyric acid
Cell Wall
gamma-Aminobutyric Acid
cell growth
Arabidopsis thaliana
cell walls
Gene Expression
gene expression
proteins
mutants
Pollen Tube
Genes
Aminobutyrates
nonprotein amino acids
Hypocotyl
Plant Development
genes
pistil
Microarray Analysis

Keywords

  • Arabidopsis thaliana
  • Cell elongation
  • Cell wall
  • GABA
  • Microarray
  • Secretory pathway

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

GABA accumulation causes cell elongation defects and a decrease in expression of genes encoding secreted and cell wall-related proteins in Arabidopsis thaliana. / Renault, Hugues; El Amrani, Abdelhak; Palanivelu, Ravishankar; Updegraff, Emily P.; Yu, Agns; Renou, Jean Pierre; Preuss, Daphne; Bouchereau, Alain; Deleu, Carole.

In: Plant and Cell Physiology, Vol. 52, No. 5, 05.2011, p. 894-908.

Research output: Contribution to journalArticle

Renault, Hugues ; El Amrani, Abdelhak ; Palanivelu, Ravishankar ; Updegraff, Emily P. ; Yu, Agns ; Renou, Jean Pierre ; Preuss, Daphne ; Bouchereau, Alain ; Deleu, Carole. / GABA accumulation causes cell elongation defects and a decrease in expression of genes encoding secreted and cell wall-related proteins in Arabidopsis thaliana. In: Plant and Cell Physiology. 2011 ; Vol. 52, No. 5. pp. 894-908.
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