Gadolinium-DTPA liposomes as a potential MRI contrast agent. Work in progress

Evan C Unger, O. Needleman, P. Cullis, C. Tilcock

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To evaluate the use of liposomes containing Gadolinium-DTPA (Gd-DTPA) as potential intravascular contrast agents, we synthesized and tested Gd-DTPA liposomes. A freeze-thaw extrusion process was used to synthesize neutral unilamellar vesicles. Using this technique, we prepared 0.4 micron vesicles with encapsulation efficiency as high as 39% for Gd-DTPA. In vitro dialysis showed that essentially 100% of the Gd-DTPA was retained with the liposomes after 72 hours of dialysis. MR imaging of in vitro samples showed concentration-dependent increase in signal intensity with Gd-DTPA liposomes. Imaging of rats after intravenous injection of Gd-DTPA liposomes showed sustained intravascular contrast enhancement of vascular structures and liver greater than free Gd-DTPA. There was no evidence of acute toxicity in rats during the imaging experiments or on follow-up of two months. Paramagnetic liposomes may be useful to enhance the vasculature, liver, and spleen.

Original languageEnglish (US)
Pages (from-to)928-932
Number of pages5
JournalInvestigative Radiology
Volume23
Issue number12
StatePublished - 1988
Externally publishedYes

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Gadolinium DTPA
Liposomes
Contrast Media
Dialysis
Unilamellar Liposomes
Liver
Intravenous Injections
Blood Vessels
Spleen

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Gadolinium-DTPA liposomes as a potential MRI contrast agent. Work in progress. / Unger, Evan C; Needleman, O.; Cullis, P.; Tilcock, C.

In: Investigative Radiology, Vol. 23, No. 12, 1988, p. 928-932.

Research output: Contribution to journalArticle

Unger, EC, Needleman, O, Cullis, P & Tilcock, C 1988, 'Gadolinium-DTPA liposomes as a potential MRI contrast agent. Work in progress', Investigative Radiology, vol. 23, no. 12, pp. 928-932.
Unger, Evan C ; Needleman, O. ; Cullis, P. ; Tilcock, C. / Gadolinium-DTPA liposomes as a potential MRI contrast agent. Work in progress. In: Investigative Radiology. 1988 ; Vol. 23, No. 12. pp. 928-932.
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