Ganglioside effects on basic fibroblast and epidermal growth factor receptors in retinal glial cells

Emmanuelle Meuillet, Gérard Crémel, David Hicks, Henri Dreyfus

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Gangliosides have long been implicated in cell growth regulation and play an important role as modulators in protein phosphorylation. In order to better understand how glycosphingolipids and growth factors interact, we examined the modulation of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) effects on retinal Muller glial cells (RMG), following modification of their GG composition. Treatment of MG cells with GG (GM1, GT1b) and asialoGM1 resulted in modifications of several aspects of cellular responses to EGF- and FGF-receptor (R) activation: mitogenesis, cell migration, tyrosine phosphorylation of the EGF-R and FGF-R and even their cellular substrates were particularly influenced by GG. Indeed GG caused modifications of EGF-R and FGF-R autophosphorylation kinetics. GG long term effects (mitogenesis and migration) correlate with short term effects (tyrosine phosphorylation) and differences in receptor tyrosine kinase signalling could explain the specificity in growth factor responses.

Original languageEnglish (US)
Pages (from-to)277-288
Number of pages12
JournalJournal of Lipid Mediators and Cell Signalling
Volume14
Issue number1-3
DOIs
StatePublished - Sep 1996

Keywords

  • Muller glial cells
  • growth factor
  • tyrosine kinase receptor

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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