Gas chromatographic determination of fentanyl and its analogues in human plasma

T. J. Gillespie, A Jay Gandolfi, R. M. Maiorino, R. W. Vaughan

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

There is currently no published chromatographic assay for the fentanyl family of analgesics. This study reports the development of a sensitive, precise, and accurate gas chromatographic method for the determination of fentanyl and its analogues in human plasma. Sensitivity attained for fentanyl and sufentanil was 0.1 ng/ml plasma with an average coefficient of variation of 4.5%. Calibration curve correlation coefficients of 0.99 or greater were consistently obtained. Average recovery of drug into the final extract exceeded 80% and was independent of fentanyl concentration. The required degree of specificity was obtained by selective extraction, use of the nitrogen/phosphorus detector, and chromatographic resolution. This method appears suitable for clinical studies of fentanyl and its derivatives in man.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalJournal of Analytical Toxicology
Volume5
Issue number3
StatePublished - 1981

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Plasma (human)
Fentanyl
Phosphorus
Assays
Gases
Calibration
Nitrogen
Derivatives
Detectors
Plasmas
plasma
Recovery
gas
drug
assay
phosphorus
calibration
nitrogen
Sufentanil
Analgesics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Gas chromatographic determination of fentanyl and its analogues in human plasma. / Gillespie, T. J.; Gandolfi, A Jay; Maiorino, R. M.; Vaughan, R. W.

In: Journal of Analytical Toxicology, Vol. 5, No. 3, 1981, p. 133-137.

Research output: Contribution to journalArticle

Gillespie, T. J. ; Gandolfi, A Jay ; Maiorino, R. M. ; Vaughan, R. W. / Gas chromatographic determination of fentanyl and its analogues in human plasma. In: Journal of Analytical Toxicology. 1981 ; Vol. 5, No. 3. pp. 133-137.
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