Gas chromatographic determination of fentanyl and its analogues in human plasma

T. J. Gillespie, A. Jay Gandolfi, R. M. Maiorino, R. W. Vaughan

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

There is currently no published chromatographic assay for the fentanyl family of analgesics. This study reports the development of a sensitive, precise, and accurate gas chromatographic method for the determination of fentanyl and its analogues in human plasma. Sensitivity attained for fentanyl and sufentanil was 0.1 ng/mL plasma with an average coefficient of variation of 4.5%. Calibration curve correlation coefficients of 0.99 or greater were consistently obtained. Average recovery of drug into the final extract exceeded 80% and was independent of fentanyl concentration. The required degree of specificity was obtained by selective extraction, use of the nitrogen/phosphorous detector, and chromatographic resolution. This method appears suitable for clinical studies of fentanyl and its derivatives in man.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalJournal of Analytical Toxicology
Volume5
Issue number3
DOIs
StatePublished - May 1981

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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