Gene activation in response to neural induction

Paul A Krieg, Aaron Zorn, Nick Ovsenek, Andrew Johnson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In order to study the molecular processes involved in the final steps of neural induction, we are investigating the transcriptional regulation of the Xenopus laevis neural cell adhesion molecule (NCAM) gene. NCAM is expressed very early during neural development and its expression is dependent on neural induction signals. Using microinjection of reporter gene constructions into fertilized frog eggs, we have shown that cloned copies of the NCAM promoter respond appropriately to neural induction signals. Examination of the Xenopus NCAM promoter reveals a number of potential regulatory elements. We have directed our attention to a small region of the promoter that is highly conserved over large evolutionary distances. This region contains an enhancer, the OZ element, and also a silencer element, the N-box. We have purified DNA binding proteins that interact specifically with both the OZ enhancer and the N-box silencer sequences. A model suggesting a role for these regulatory factors during neural induction is presented.

Original languageEnglish (US)
Pages (from-to)377-383
Number of pages7
JournalCellular and Molecular Biology Research
Volume39
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Neural Cell Adhesion Molecules
Transcriptional Activation
Genes
Chemical activation
Transcriptional Silencer Elements
Zygote
Xenopus laevis
DNA-Binding Proteins
Microinjections
Xenopus
Reporter Genes
Genetic Promoter Regions
Anura

Keywords

  • NCAM gene
  • Silencer element
  • Transcription factors
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Krieg, P. A., Zorn, A., Ovsenek, N., & Johnson, A. (1993). Gene activation in response to neural induction. Cellular and Molecular Biology Research, 39(4), 377-383.

Gene activation in response to neural induction. / Krieg, Paul A; Zorn, Aaron; Ovsenek, Nick; Johnson, Andrew.

In: Cellular and Molecular Biology Research, Vol. 39, No. 4, 1993, p. 377-383.

Research output: Contribution to journalArticle

Krieg, PA, Zorn, A, Ovsenek, N & Johnson, A 1993, 'Gene activation in response to neural induction', Cellular and Molecular Biology Research, vol. 39, no. 4, pp. 377-383.
Krieg, Paul A ; Zorn, Aaron ; Ovsenek, Nick ; Johnson, Andrew. / Gene activation in response to neural induction. In: Cellular and Molecular Biology Research. 1993 ; Vol. 39, No. 4. pp. 377-383.
@article{d28480cfc36442678b391cd627a78a35,
title = "Gene activation in response to neural induction",
abstract = "In order to study the molecular processes involved in the final steps of neural induction, we are investigating the transcriptional regulation of the Xenopus laevis neural cell adhesion molecule (NCAM) gene. NCAM is expressed very early during neural development and its expression is dependent on neural induction signals. Using microinjection of reporter gene constructions into fertilized frog eggs, we have shown that cloned copies of the NCAM promoter respond appropriately to neural induction signals. Examination of the Xenopus NCAM promoter reveals a number of potential regulatory elements. We have directed our attention to a small region of the promoter that is highly conserved over large evolutionary distances. This region contains an enhancer, the OZ element, and also a silencer element, the N-box. We have purified DNA binding proteins that interact specifically with both the OZ enhancer and the N-box silencer sequences. A model suggesting a role for these regulatory factors during neural induction is presented.",
keywords = "NCAM gene, Silencer element, Transcription factors, Xenopus",
author = "Krieg, {Paul A} and Aaron Zorn and Nick Ovsenek and Andrew Johnson",
year = "1993",
language = "English (US)",
volume = "39",
pages = "377--383",
journal = "Cellular and Molecular Biology Research",
issn = "0968-8773",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Gene activation in response to neural induction

AU - Krieg, Paul A

AU - Zorn, Aaron

AU - Ovsenek, Nick

AU - Johnson, Andrew

PY - 1993

Y1 - 1993

N2 - In order to study the molecular processes involved in the final steps of neural induction, we are investigating the transcriptional regulation of the Xenopus laevis neural cell adhesion molecule (NCAM) gene. NCAM is expressed very early during neural development and its expression is dependent on neural induction signals. Using microinjection of reporter gene constructions into fertilized frog eggs, we have shown that cloned copies of the NCAM promoter respond appropriately to neural induction signals. Examination of the Xenopus NCAM promoter reveals a number of potential regulatory elements. We have directed our attention to a small region of the promoter that is highly conserved over large evolutionary distances. This region contains an enhancer, the OZ element, and also a silencer element, the N-box. We have purified DNA binding proteins that interact specifically with both the OZ enhancer and the N-box silencer sequences. A model suggesting a role for these regulatory factors during neural induction is presented.

AB - In order to study the molecular processes involved in the final steps of neural induction, we are investigating the transcriptional regulation of the Xenopus laevis neural cell adhesion molecule (NCAM) gene. NCAM is expressed very early during neural development and its expression is dependent on neural induction signals. Using microinjection of reporter gene constructions into fertilized frog eggs, we have shown that cloned copies of the NCAM promoter respond appropriately to neural induction signals. Examination of the Xenopus NCAM promoter reveals a number of potential regulatory elements. We have directed our attention to a small region of the promoter that is highly conserved over large evolutionary distances. This region contains an enhancer, the OZ element, and also a silencer element, the N-box. We have purified DNA binding proteins that interact specifically with both the OZ enhancer and the N-box silencer sequences. A model suggesting a role for these regulatory factors during neural induction is presented.

KW - NCAM gene

KW - Silencer element

KW - Transcription factors

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0027716985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027716985&partnerID=8YFLogxK

M3 - Article

VL - 39

SP - 377

EP - 383

JO - Cellular and Molecular Biology Research

JF - Cellular and Molecular Biology Research

SN - 0968-8773

IS - 4

ER -