Gene activation in response to neural induction

Paul A Krieg, Aaron Zorn, Nick Ovsenek, Andrew Johnson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

In order to study the molecular processes involved in the final steps of neural induction, we are investigating the transcriptional regulation of the Xenopus laevis neural cell adhesion molecule (NCAM) gene. NCAM is expressed very early during neural development and its expression is dependent on neural induction signals. Using microinjection of reporter gene constructions into fertilized frog eggs, we have shown that cloned copies of the NCAM promoter respond appropriately to neural induction signals. Examination of the Xenopus NCAM promoter reveals a number of potential regulatory elements. We have directed our attention to a small region of the promoter that is highly conserved over large evolutionary distances. This region contains an enhancer, the OZ element, and also a silencer element, the N-box. We have purified DNA binding proteins that interact specifically with both the OZ enhancer and the N-box silencer sequences. A model suggesting a role for these regulatory factors during neural induction is presented.

Original languageEnglish (US)
Pages (from-to)377-383
Number of pages7
JournalCellular and Molecular Biology Research
Volume39
Issue number4
Publication statusPublished - 1993
Externally publishedYes

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Keywords

  • NCAM gene
  • Silencer element
  • Transcription factors
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Krieg, P. A., Zorn, A., Ovsenek, N., & Johnson, A. (1993). Gene activation in response to neural induction. Cellular and Molecular Biology Research, 39(4), 377-383.