Gene amplification affecting O6-alkylguanine-DNA alkyltransferase activity is not detected in nitrosourea resistant or sensitive human cell lines

Nicholas S. Vlahos, Bernard W Futscher, Nancy K. Hora, Jeffrey M. Trent, Leonard C. Erickson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An attempt was made to characterize the genetic regulation of the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) in the absence of the cloned gene. Four human cell lines, differing in AGT activity from very proficient to essentially absent, were assayed for gene amplification as a possible mediator of the methylation repair phenotype (Mer+, AGT activity and MER-, no AGT activity) using in-gel DNA renaturation and G-banded karyotype analysis. The former technique allows subsequent analysis of amplification units and cloning of observed amplified DNA fragments, a hopeful approach to the isolation of the human AGT gene. Within the sensitivities of the techniques, no correlation between AGT activity and gene amplification was observed in the four cell lines tested.

Original languageEnglish (US)
Pages (from-to)479-483
Number of pages5
JournalCarcinogenesis
Volume11
Issue number3
StatePublished - Mar 1990
Externally publishedYes

Fingerprint

Gene Amplification
Amplification
DNA
Cells
Gene
Cell Line
Line
Cell
Medical Genetics
Karyotype
DNA Repair
Methylation
Genes
Repair
Organism Cloning
Gels
Phenotype
Mediator
Cloning
Isolation

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Gene amplification affecting O6-alkylguanine-DNA alkyltransferase activity is not detected in nitrosourea resistant or sensitive human cell lines. / Vlahos, Nicholas S.; Futscher, Bernard W; Hora, Nancy K.; Trent, Jeffrey M.; Erickson, Leonard C.

In: Carcinogenesis, Vol. 11, No. 3, 03.1990, p. 479-483.

Research output: Contribution to journalArticle

Vlahos, Nicholas S. ; Futscher, Bernard W ; Hora, Nancy K. ; Trent, Jeffrey M. ; Erickson, Leonard C. / Gene amplification affecting O6-alkylguanine-DNA alkyltransferase activity is not detected in nitrosourea resistant or sensitive human cell lines. In: Carcinogenesis. 1990 ; Vol. 11, No. 3. pp. 479-483.
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