Gene expression profiles during the initial phase of salt stress in rice

S. Kawasaki, C. Borchert, M. Deyholos, H. Wang, S. Brazille, K. Kawai, David W Galbraith, H. J. Bohnert

Research output: Contribution to journalArticle

716 Citations (Scopus)

Abstract

Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defense-related functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.

Original languageEnglish (US)
Pages (from-to)889-905
Number of pages17
JournalPlant Cell
Volume13
Issue number4
DOIs
StatePublished - 2001

Fingerprint

Transcriptome
Gene expression
salt stress
Salts
salts
rice
gene expression
Salinity
Microarrays
salinity
Shock
Down-Regulation
aquaporins
protein metabolism
nucleic acid hybridization
Aquaporins
Photosynthesis
Transcription
transcription (genetics)
protein synthesis

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., ... Bohnert, H. J. (2001). Gene expression profiles during the initial phase of salt stress in rice. Plant Cell, 13(4), 889-905. https://doi.org/10.1105/tpc.13.4.889

Gene expression profiles during the initial phase of salt stress in rice. / Kawasaki, S.; Borchert, C.; Deyholos, M.; Wang, H.; Brazille, S.; Kawai, K.; Galbraith, David W; Bohnert, H. J.

In: Plant Cell, Vol. 13, No. 4, 2001, p. 889-905.

Research output: Contribution to journalArticle

Kawasaki, S, Borchert, C, Deyholos, M, Wang, H, Brazille, S, Kawai, K, Galbraith, DW & Bohnert, HJ 2001, 'Gene expression profiles during the initial phase of salt stress in rice', Plant Cell, vol. 13, no. 4, pp. 889-905. https://doi.org/10.1105/tpc.13.4.889
Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K et al. Gene expression profiles during the initial phase of salt stress in rice. Plant Cell. 2001;13(4):889-905. https://doi.org/10.1105/tpc.13.4.889
Kawasaki, S. ; Borchert, C. ; Deyholos, M. ; Wang, H. ; Brazille, S. ; Kawai, K. ; Galbraith, David W ; Bohnert, H. J. / Gene expression profiles during the initial phase of salt stress in rice. In: Plant Cell. 2001 ; Vol. 13, No. 4. pp. 889-905.
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