Gene-specific DNA interstrand cross-links produced by nitrogen mustard in the human tumor cell line Colo320HSR.

B. W. Futscher, R. O. Pieper, W. S. Dalton, L. C. Erickson

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Genomic and gene-specific DNA interstrand cross-links produced by nitrogen mustard (HN2) were measured in the human tumor cell line Colo320HSR. Following exposures that produced greater than or equal to 1 log cell kill, it was found that HN2-induced DNA interstrand cross-links were produced and processed in a heterogeneous fashion within the genome. Cross-links were detected in the amplified, overexpressed c-myc oncogene, whereas in the weakly expressed N-ras gene and the nontranscribed, high copy number alpha-satellite sequence (of chromosome 20), cross-links were not detected. The cross-links in the c-myc oncogene disappeared more rapidly than total genomic cross-links. These results suggest that HN2-induced DNA interstrand cross-links are produced and processed in the genome in a nonrandom fashion.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalCell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
Volume3
Issue number4
StatePublished - Apr 1992
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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